Products for Epigenetics, Chromatin Biology
& Transcriptional Regulation

At Active Motif, we develop innovative cell biology-based research tools and biocomputing resources that help researchers elucidate the function, regulation and interactions of the genes and their encoded proteins. Our goal is to provide our customers with high-quality products complemented by superior service & support.

New & Notable Products

• New Antibodies for 5-formylcytosine & 5-carboxylcytosine

  DNA methylation is an important part of mechanisms regulating genome accessibility and function, and Active Motif offers a number of kits and reagents to study the different forms of methylated DNA. In addition to 5-methylcytosine and 5-hydroxymethylcytosine methylation, we now offer highly specific and well validated antibodies for the study of two novel forms of methylation, 5-formylcytosine and 5‑carboxylcytosine.

• New EpiShear™ Multi-Sample Sonicator

  The new EpiShear™ Multi-Sample Sonicator is a high-intensity cup horn sonicator that can shear up to eight vials (20 µl - 1.2 ml sample per vial) of chromatin or DNA simultaneously for use in chromatin immunoprecipitation (ChIP), DNA methylation studies and Next-Gen sequencing. More >

• EpiShear™ Cooled Sonication Platform Improves Reproducibility

  The new EpiShear™ Cooled Sonication Platform improves sample-to-sample reproducibility when using probe sonicators by keeping the sample cold while enabling the probe to be positioned at the same depth in the sample every time. More >

• New Antibodies for the Study of 3-methylcytosine

  Active Motif is once again the first company to offer you antibodies for novel DNA modifications. Our new antibodies to 3-Methylcytosine will help you study the function of this form of DNA methylation. More >

• New Hydroxymethyl Collector™ Kit for Biotin-based 5-hmC Capture

  Active Motif continues to expand its portfolio of products to study 5-hydroxymethylcytosine. Our new Hydroxymethyl Collector™ Kit is able to chemically label 5-hmC residues for specific enrichment of DNA fragments containing 5-hydroxymethylcytosine. A biotin-streptavidin capture method is used to isolate the labeled 5-hmC residues. More >

• Dounce Homogenizer for Better Chromatin and Lysate Preparation

  To help improve the quality of your sample preparations for chromatin immunoprecipitation experiments or for regular extraction of cell or tissue lysates, Active Motif now offers a Dounce Homogenizer. There are two sizes available for either small or large scale sample preparations. More >

• Antibodies for the Study of Transcriptional Regulation

  Active Motif has released a number of new antibodies for the study of transcriptional regulation including CtIP, Notch1, PBX, PBX2 and PCL2.

• Validated qPCR Primer Sets for use as ChIP Controls

  Active Motif offers a large variety of human- and mouse-specific ChIP Control qPCR Primer Sets for many of the more common ChIP targets. These positive and negative controls have been validated and proven to work in both qPCR and endpoint PCR, and are used regularly by our Epigenetics Services division. More >

• Protease Inhibitor Cocktail Improves Cell Lysis and Extraction

  Active Motif now sells Protease Inhibitor Cocktail. The Protease Inhibitor Cocktail is a combination of six different cysteine, serine, acid and aminopeptidase inhibitors that can be used to protect cell lysates from proteolytic degradation during sample preparation. More >

• PvuRts1 I Enzyme for Direct Differentiation of 5-mC and 5-hmC

  PvuRts1 I restriction enzyme is the first enzyme capable of directly differentiating between 5-mC and 5-hmC DNA. PvuRts1 I only cleaves 5-hydroxymethylcytosine DNA, in either its glucosylated or non-glucosylated form, while leaving 5-methylcytosine and unmethylated DNA untouched. More >

• New β-Glucosyltransferase Enzyme for 5-hmC DNA Modification

  β-Glucosyltransferase enables modification of 5-hydroxymethylcytosine DNA through the addition of glucose, generating glucosyl-5-hmC DNA. This modified glucosyl-5-hmC DNA can be quantified, or studied using glucosyl-sensitive restriction enzymes. More >

• MeDIP Kit Now Available for 5-methylcytosine DNA Enrichment

  Active Motif now offers a MeDIP Kit for the isolation of single-stranded DNA containing 5-methylcytosine. This kit can be used alone, or in combination with our hMeDIP Kit. More >

• New hMeDIP Kit for Specific Enrichment of 5-hmC DNA

  To better understand the role of 5-hydroxymethylcytosine (5-hmC) DNA methylation, Active Motif introduces its hMeDIP Kit, which specifically enriches for 5-hmC DNA fragments. Unlike traditional MeDIP, the hMeDIP Kit can be used with double-stranded DNA, which greatly simplifies downstream analysis. More >

• Get Analysis-ready DNA with the Chromatin IP DNA Purification Kit

  Quickly and easily clean up your ChIP DNA with the Chromatin IP DNA Purification Kit. Remove contaminants that interfere with downstream analysis (microarray, qPCR, Next-Gen sequencing). The kit can also prepare DNA for use with our hMeDIP, MethylCollector™ Ultra and UnMethylCollector™ Kits.

• Methylated DNA Standard Kit for the Study of DNA Methylation

  Try our new Methylated DNA Standard Kit for experiments examining the different types of DNA methylation. It includes DNA derived from the APC promoter that has no methylation, 5-methylcytosine methylation or 5-hydroxymethylcytosine methylation. Also included are specific primers to aid in detection by PCR.

• New Recombinant Methylated and Acetylated Histones

  Active Motif has expanded its line of recombinant histones to include mono-, di- and trimethylated proteins for histone H3 Lys14, Lys18, Lys23 and histone H4 Lys5 and Lys16. We have also added recombinant histone H3 acetyl Lys23. Click here to see a complete listing of the over 40 recombinant methylated and acetylated histones now available.

• MODified™ Histone Peptide Array and FREE Software

  Screen 384 different histone modification combinations in a single experiment. The MODified™ Histone Peptide Array allows you to screen antibodies or enzymes for interactions with histones and their post-translational modifications on the N-terminal tails of H2A, H2B, H3 and H4. More >