Active Motif,
Tools to analyze nuclear function,
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Cas9 Antibody (mAb)

Active Motif introduces its new Cas9
antibody for use in monitoring the
targeting efficiency and specificity
of Cas9 endonuclease.

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Cas9 anbibody (mAb)

Histone Analysis Products

Our products to study histones & histone modifications
include MODified™ arrays, Histone Modification ELISAs,
recombinant histones, HAT/HDAC assays and enzymes,
kits for histone purification & chromatin assembly, as well
as over 100 antibodies to specific histone modifications.

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Histone Analysis Products

Ready-to-use ChIP Columns

The Protein G Agarose Columns are supplied loaded with
pre-washed protein G agarose beads. Not only does this
save time and effort, but because all incubation and wash steps
are performed on the column, sample loss is eliminated,
resulting in much better sample-to-sample reproducibility.

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Ready-to-use Protein G Agarose ChIP Columns

5-Hydroxymethylcytosine Research

Active Motif has a variety of products to help study
5-hmC and 5-mC, including h-MeDIP & MeDIP Kits,
a unique methylation-sensitive restriction enzyme,
β-Glucosyltransferase enzyme, as well as several
MeDIP-validated 5-hmC and 5-mC antibodies.

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5-Hydroxymethylcytosine Research

Luminex® Multiplex Assays for Histone

Modifications & Transcription Factors

The Histone PTM and Transcription Factor Multiplex Kits can quantify
the levels of different histone modifications, or the binding activities of
NFκB or AP-1 family members, all in the in the same sample.

 Histone Modification Assays   Transcription Factor Assays 
Luminex Multiplex Histone Modification and Transcription Factor Assays

EpiShear™ Probe Sonicator

The EpiShear™ Probe Sonicator is a compact unit that
is ideal for shearing chromatin for ChIP and DNA
methylation studies. The programmable device gives
you complete control over pulse amplitude and duration,
so you can use your optimized settings every time.

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EpiShear Probe Sonicator

Antibodies for ChIP-Seq from Active Motif

New & Notable Products for Epigenetics,
Chromatin Biology and Gene Regulation

  • New Recombinant Nucleosomes for use in Enzymatic Assays and Chromatin Assembly

    Active Motif's Recombinant Nucleosomes offer a more biologically relevant choice of substrate when compared to histone proteins alone or synthetic peptides for use in drug discovery screening, analyzing enzyme kinetics or monitoring changes in histone modifications. Each recombinant nucleosome is comprised of octamers of unmodified core histone proteins (H2A, H2B, H3 and H4) bound by DNA. Biotin-tagged Nucleosomes are also available for pull-down or binding assays. More >

  • New ChIP-Exonuclease Kit for High-Resolution ChIP Analysis

    ChIP-exo provides a high-resolution method for mapping transcription factor binding sites genome-wide. It utilizes exonuclease-mediated digestion of DNA during the immunoprecipitation protocol to remove non-cross-linked DNA and increase binding site resolution to within 20-95 bp reads. This makes ChIP-exo ideal for mapping transcription factor binding sites, performing discovery-based studies or evaluation of SNP or mutation effects. More >

  • ChIP-Bisulfite-Sequencing for Methylation Profiles of your ChIP DNA

    ChIP-Bis-Seq enables direct interrogation of both chromatin and DNA methylation profiles on the same sample. Evaluate allele-specific difference, or gene regulatory effects with reduced sequencing costs by focusing on specific genomic regions. More >

  • Easily Isolate Mononucleosomes or Oligonucleosomes from your Samples

    Easily isolate mononucleosomes or oligonucleosomes from your samples using Active Motif's new Nucleosome Preparation Kit. Nucleosomes offer a more physiologically relevant substrate for histone post-translational studies, enzyme kinetic analysis and inhibitor screening studies. More >

  • Global DNA Methylation Assay to Quantify 5-mC Levels in DNA

    Active Motif's new Global DNA Methylation Assay – LINE-1 Kit is a simple, easy-to-use ELISA based assay for high throughput quantitation of global 5-methylcytosine (5-mC) levels, but is unique in that it utilizes LINE-1 methylation as a surrogate for measuring global DNA methylation. This format improves specificity of capture and sensitivity, enabling quantitation of as low as 0.5% methylation and detection of methylation from as little as 10 ng of input DNA. More >

  • Cas9 Antibody for Use in CRISPR/Cas9 Gene Editing

    Active Motif now offers a Cas9 monoclonal antibody validated for use in immunoprecipitation, immunofluorescence and Western blot experiments. While Cas9 is a component of the CRISPR/Cas prokaryotic immune system that provides protection against mobile genetic elements (viruses, transposable elements, plasmids etc.), CRISPR/Cas is used by scientists as a useful tool to insert or delete sequences at any specific location in a cell's genome. This antibody can be used to verify expression of Cas9. More >

  • Activators and Inhibitors for Epigenetic Research

    Active Motif now offers a collection of Epigenetic Activators and Inhibitors. These include small molecules that target HATs, HDACs, HMTs, HDMs, Sirtuins and DNA methylation. These epigenetic modulators represent an important tool for researchers performing both mechanistic and drug discovery assays. More >

  • PBMC ChIP Kit Offers a Complete Solution for ChIP from B and T Cells

    PBMCs (peripheral blood mononuclear cells), like other primary cells, are notoriously difficult to lyse which presents a challenge for chromatin preparation for ChIP. Active Motif's new ChIP-IT® PBMC Kit is the first commercial kit available that offers a complete solution for the ChIP assay workflow by providing reagents and protocols for chromatin preparation, ChIP and DNA purification that have been specifically formulated to work with PBMCs such as lymphocytes (T cells, B cells and NK cells) and monocytes. More >

  • NOMe-Seq – A Top Ten Innovations 2013 winner by The Scientist

    NOMe-Seq is the first commercially available assay to study nucleosome occupancy and CpG methylation status simultaneously on the same DNA strand. More >

  • Rapid Bisulfite Conversion Kit for Single-nucleotide DNA Methylation Analysis, Including Positive Control Primers for Validation

    Active Motif's Bisulfite Conversion Kit is a rapid 1.5 hour conversion protocol to convert unmethylated cytosines into uracil for single-nucleotide DNA methylation analysis. More >

  • Post-transcriptional Regulation Mediated by miRNA-3´UTR Interactions

    While gene regulation studies have historically focused on events that influence transcription, post-transcriptional regulation plays an equally important role. The binding of miRNAs to the 3´UTRs of transcribed mRNAs affect both the transcript’s stability and rate of translation. To study miRNA-3´UTR interactions, Active Motif offers over 12,000 LightSwitch™ 3´UTR Reporter constructs, as well as over 900 synthetic miRNA targets, and 1000 miRNA mimics & inhibitors. More >

  • Obtain ChIP-Seq Data from FFPE Tissue Samples

    Active Motif's new ChIP-IT® FFPE Chromatin Preparation Kit and ChIP-IT® FFPE Kit are the first kits available to perform ChIP analysis of formalin-fixed paraffin-embedded (FFPE) tissue material. Due to the limited size and harsh fixation conditions used to prepare FFPE slides and tissue blocks, these samples have previously not been useful in ChIP. Active Motif's new assays are the only kits with the sensitivity required to obtain ChIP-Seq quality DNA from FFPE tissues. More >

  • New Luminex® Multiplex Assay to Screen Histone PTMs

    Active Motif has partnered with Luminex® to develop a 96-well plate-based multiplex assay for the analysis of histone PTMs. The Histone PTM Multiplex Kit enables simultaneous analysis of up to 13 different Histone H3 modifications within the same well using only nanogram quantities of sample. More >

  • More Recombinant Methyltransferases and Demethylases to Choose From

    Active Motif has recently expanded its offering of high-quality Recombinant Histone Methyltransferases (HMTs) and Histone Demethylases (HDMs), including newly added PRC2 complexes (including complexes with EZH2 point mutations), and additional Jumonji, arginine methyltransferases and arginine demethylases. The enzymes are suitable for use in studies of enzyme kinetics, profiling and screening of inhibitors, and drug discovery and development. More >

  • Over 30,000 Pre-Cloned Regulatory Elements for Reporter Assays

    The LightSwitch™ Luciferase Assay System includes over 18,000 promoters and 12,000 3´UTRs cloned from the human genome and available as transfection-ready LightSwitch luciferase reporter vectors. Other LightSwitch products include Pathway Reporter Stable Cell Lines, Synthetic miRNA Targets, and miRNA Mimics and Inhibitors. More >

  • MODified™ Protein Domain Binding Kit to Assay Specificity of Protein Reading Domains

    The MODified™ Protein Domain Binding Kit is designed to be used in combination with Active Motif's MODified Histone Peptide Array to screen His-tagged protein domains for interaction with histone and their post-translational modifications. The kit contains optimized buffers and reagents for analysis of protein domain binding specificity along with HRP-conjugated antibodies and ECL reagents chemiluminescent detection. For added convenience, a positive control tudor domain protein is also provided.More >

  • Novel Stem Cell Dye for the Identification of Live Stem Cells

    Stem Cell CDy1 Dye is designed for selective staining of live stem cells. The CDy1 dye can be used for identification and isolation of various cell types including ESCs, iPSCs, neural stem cells and cancer stem cells by fluorescence imaging or FACS analysis. More >

  • ChIP-IT® ChIP-Seq Kit for Whole-genome ChIP Analysis

    Active Motif now offers a complete workflow for ChIP-Seq experiments with our new ChIP-IT® ChIP-Seq Kit. The Kit provides proven reagents and protocols for successful ChIP-Seq results. More >

  • High Sensitivity ChIP Kit

    Obtain high quality ChIP DNA when working with low abundance transcription factor targets, antibodies with sub-optimal binding affinities or when using as few as 1,000 cells per IP with Active Motif's ChIP-IT® High Sensitivity Kit. More >

  • Study NFκB Activation/Translocation via IκBα Phosphorylation

    Inactive NFκB dimers are sequestered in the cytoplasm by the IκB family of inhibitor proteins, which mask the NFκB nuclear localization signals. Phosphorylation of IκBα in response to various external stimuli enables activated NFκB to translocate to the nucleus and regulate a variety of genes. The FunctionELISA™ IκBα Kit is ideal for studying events that lead to IκBα phosphorylation. More >

  • Ready-to-Use Protein G Agarose Columns for ChIP

    Active Motif's Protein G Agarose Columns contain pre-washed Protein G agarose loaded into columns and ready to use for ChIP. Simply add the ChIP reaction to the column and go. Wash steps are also performed in the column which makes the process faster and also ensures complete sample recovery for better reproducibility. More >

  • Recombinant Bromodomain Proteins

    Active Motif has just introduced a large number of high-quality Recombinant Bromodomain proteins for use in studies of bromodomain function in transcriptional regulation and chromatin remodeling. More >

MotifVations Newsletter

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Careers at Active Motif

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motivated individuals to join
our sales & research teams.
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Webinars

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Epigenetics News

View recently published, notable papers on epigenetics. More >

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