DNA Methylation Antibodies
Active Motif offers a large variety of highly
validated antibodies for the study of
DNA methylation in all its forms, including
antibodies against 5-mC, 5-hmC, 5-fC,
5-caC, 3-mC, and a number of DNMTs.
Active Motif now offers a collection of Epigenetic Activators and Inhibitors. These include small molecules that target HATs, HDACs, HMTs, HDMs, Sirtuins and DNA methylation. These epigenetic modulators represent an important tool for researchers performing both mechanistic and drug discovery assays. More >
PBMCs (peripheral blood mononuclear cells), like other primary cells, are notoriously difficult to lyse which presents a challenge for chromatin preparation for ChIP. Active Motif's new ChIP-IT® PBMC Kit is the first commercial kit available that offers a complete solution for the ChIP assay workflow by providing reagents and protocols for chromatin preparation, ChIP and DNA purification that have been specifically formulated to work with PBMCs such as lymphocytes (T cells, B cells and NK cells) and monocytes. More >
While gene regulation studies have historically focused on events that influence transcription, post-transcriptional regulation plays an equally important role. The binding of miRNAs to the 3´UTRs of transcribed mRNAs affect both the transcript’s stability and rate of translation. To study miRNA-3´UTR interactions, Active Motif offers over 12,000 LightSwitch™ 3´UTR Reporter constructs, as well as over 900 synthetic miRNA targets, and 1000 miRNA mimics & inhibitors. More >
Active Motif's new ChIP-IT® FFPE Chromatin Preparation Kit and ChIP-IT® FFPE Kit are the first kits available to perform ChIP analysis of formalin-fixed paraffin-embedded (FFPE) tissue material. Due to the limited size and harsh fixation conditions used to prepare FFPE slides and tissue blocks, these samples have previously not been useful in ChIP. Active Motif's new assays are the only kits with the sensitivity required to obtain ChIP-Seq quality DNA from FFPE tissues. More >
Active Motif has partnered with Luminex® to develop a 96-well plate-based multiplex assay for the analysis of histone PTMs. The Histone PTM Multiplex Kit enables simultaneous analysis of up to 13 different Histone H3 modifications within the same well using only nanogram quantities of sample. More >
Active Motif has recently expanded its offering of high-quality Recombinant Histone Methyltransferases (HMTs) and Histone Demethylases (HDMs), including newly added PRC2 complexes (including complexes with EZH2 point mutations), and additional Jumonji, arginine methyltransferases and arginine demethylases. The enzymes are suitable for use in studies of enzyme kinetics, profiling and screening of inhibitors, and drug discovery and development. More >
The LightSwitch™ Luciferase Assay System includes over 18,000 promoters and 12,000 3´UTRs cloned from the human genome and available as transfection-ready LightSwitch luciferase reporter vectors. Other LightSwitch products include Pathway Reporter Stable Cell Lines, Synthetic miRNA Targets, and miRNA Mimics and Inhibitors. More >
The MODified™ Protein Domain Binding Kit is designed to be used in combination with Active Motif's MODified Histone Peptide Array to screen His-tagged protein domains for interaction with histone and their post-translational modifications. The kit contains optimized buffers and reagents for analysis of protein domain binding specificity along with HRP-conjugated antibodies and ECL reagents chemiluminescent detection. For added convenience, a positive control tudor domain protein is also provided.More >
Stem Cell CDy1 Dye is designed for selective staining of live stem cells. The CDy1 dye can be used for identification and isolation of various cell types including ESCs, iPSCs, neural stem cells and cancer stem cells by fluorescence imaging or FACS analysis. More >
Obtain high quality ChIP DNA when working with low abundance transcription factor targets, antibodies with sub-optimal binding affinities or when using as few as 1,000 cells per IP with Active Motif's ChIP-IT® High Sensitivity Kit. More >
Inactive NFκB dimers are sequestered in the cytoplasm by the IκB family of inhibitor proteins, which mask the NFκB nuclear localization signals. Phosphorylation of IκBα in response to various external stimuli enables activated NFκB to translocate to the nucleus and regulate a variety of genes. The FunctionELISA™ IκBα Kit is ideal for studying events that lead to IκBα phosphorylation. More >
Active Motif's Protein G Agarose Columns contain pre-washed Protein G agarose loaded into columns and ready to use for ChIP. Simply add the ChIP reaction to the column and go. Wash steps are also performed in the column which makes the process faster and also ensures complete sample recovery for better reproducibility. More >
Active Motif has just introduced a large number of high-quality Recombinant Bromodomain proteins for use in studies of bromodomain function in transcriptional regulation and chromatin remodeling. More >
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