DNA Methylation Antibodies
Active Motif offers a large variety of highly
validated antibodies for the study of
DNA methylation in all its forms, including
antibodies against 5-mC, 5-hmC, 5-fC,
5-caC, 3-mC, and a number of DNMTs.
The LightSwitch™ Luciferase Assay System includes over 18,000 promoters and 12,000 3´UTRs cloned from the human genome and available as transfection-ready LightSwitch luciferase reporter vectors. Find Your Clones >
Stem Cell CDy1 Dye is designed for selective staining of live stem cells. The CDy1 dye can be used for identification and isolation of various cell types including ESCs, iPSCs, neural stem cells and cancer stem cells by fluorescence imaging or FACS analysis. More >
Obtain high quality ChIP DNA when working with low abundance transcription factor targets, antibodies with sub-optimal binding affinities or when using as few as 1,000 cells per IP with Active Motif's ChIP-IT® High Sensitivity Kit. More >
Simplify ChIP qPCR analysis with Active Motif's ChIP-IT® qPCR Analysis Kit to normalize data according to primer pair efficiency, the amount of chromatin per reaction and the resuspension volume of the ChIP DNA. The easy-to-use spreadsheet automatically calculates and graphs data to make it easier to compare results between different samples and experiments. More >
Active Motif has just introduced a large number of high-quality Recombinant Bromodomain proteins for use in studies of bromodomain function in transcriptional regulation and chromatin remodeling. More >
It is important to use appropriate controls when studying the different types of DNA methylation. Our Methylated DNA Standard Kit can help you validate experiments examining 5-methylcytosine (5-mC) and 5-hydroxymethylcytosine (5-mC) methylation, while our 5-Carboxylcytosine DNA Standard Kit confirms 5-carboxylcytosine (5-caC) methylation. We also offer Fully Methylated Jurkat DNA and a wide assortment of DNA methylation / demethylation antibodies. More >
Active Motif now offers ChIP-IT® Control qPCR Kits for the analysis of human, mouse or rat chromatin samples. These kits will confirm the chromatin preparation and IP procedure worked properly and allow you to confirm the quality of your test antibody and qPCR primer sets. The ChIP-IT Control qPCR Kits should be used in combination with Active Motif's ChIP-IT Express or ChIP-IT Express Enzymatic Kits. For more details on all of our ChIP products, please click here.
For scientists requiring validated antibodies for use in ChIP-Seq and ChIP-on-chip, Active Motif is now in the process of re-evaluating its large selection of histone modifying and transcription factor antibodies. ChIP-Seq/ChIP-chip Antibody List.
DNA methylation is an important part of mechanisms regulating genome accessibility and function, and Active Motif offers a number of kits and reagents to study the different forms of methylated DNA. In addition to 5-methylcytosine and 5-hydroxymethylcytosine methylation, we now offer highly specific and well validated antibodies for the study of two novel forms of methylation, 5-formylcytosine and 5‑carboxylcytosine.
EpiShear™ sonication products were designed to ensure you get high-quality sheared chromatin or DNA every sample. The EpiShear Multi-Sample Sonicator is a high-intensity cup horn sonicator that can process up to 8 samples simultaneously, while the EpiShear Probe Sonicator provides you the flexibility to shear a larger range of sample sizes. It works well in conjunction with the EpiShear Cooled Sonication Platform, which improves sample-to-sample reproducibility by positioning the probe at the same depth in the sample every time. EpiShear Overview >
Active Motif continues to expand its portfolio of products to study 5-hydroxymethylcytosine. Our new Hydroxymethyl Collector™ Kit is able to chemically label 5-hmC residues for specific enrichment of DNA fragments containing 5-hydroxymethylcytosine. A biotin-streptavidin capture method is used to isolate the labeled 5-hmC residues. More >
Active Motif offers a large variety of human- and mouse-specific ChIP Control qPCR Primer Sets for many of the more common ChIP targets. These positive and negative controls have been validated and proven to work in both qPCR and endpoint PCR, and are used regularly by our Epigenetic Services division. More >
Active Motif offers its MeDIP Kit for the isolation of single-stranded DNA fragments that contain 5-methylcytosine (5-mC). It can be used alone, or in combination with our hMeDIP Kit, which specifically enriches for 5-hydroxymethylcytosine (5-hmC) DNA fragments. The hMeDIP Kit has the advantage that, unlike traditional MeDIP, it can also enrich for double-stranded DNA, which greatly simplifies downstream analysis. Methylated DNA Enrichment Overview >
Quickly and easily clean up your ChIP DNA with the Chromatin IP DNA Purification Kit. Remove contaminants that interfere with downstream analysis (microarray, qPCR, Next-Gen sequencing). The kit can also prepare DNA for use with our hMeDIP, MethylCollector™ Ultra and HypoMethylCollector™ Kits.
Over 18,000 human promoters and 12,000 3´UTRs are now available as transfection-ready LightSwitch™ luciferase reporters.
Active Motif has sponsored the March Focus issue of Nature Structural & Molecular Biology, so you can download it for free.
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