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High Sensitivity ChIP for the Most

Challenging Targets and Samples

ChIP-IT® High Sensitivity provides great ChIP results, even with
difficult-to-ChIP materials, such as low abundance transcription
factors, low-affinity antibodies or limited amounts of starting material.

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DNA Methylation Antibodies

Active Motif offers a large variety of highly
validated antibodies for the study of
DNA methylation in all its forms, including
antibodies against 5-mC, 5-hmC, 5-fC,
5-caC, 3-mC, and a number of DNMTs.

 See our DNA Methylation Antibodies 
DNA Methylation Antibodies

Transcription Factor Assays

TransAM® Kits are DNA-binding assays that facilitate
the study of transcription factor activation in mammalian
tissue and cell culture extracts. TransAM has
been cited in over 800 papers, making
it the overwhelming method of choice.

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TransAM DNA-binding ELISAs

Study miRNA-3´UTR Interactions

The LightSwitch™ 3´UTR Reporter Collection includes
over 12,000 3´UTRs cloned from the human genome into
ready-to-transfect reporter vectors. Combined with our large
collections of miRNA Mimics and Inhibitors, you have
everything needed to study miRNA-3´UTR interactions.

 Learn More 
LightSwitch 3'UTR-miRNA Interactions

MODified™ Histone Peptide Array

Screen antibodies, enzymes and proteins for
binding interactions with histones and 59 specific
acetylation, methylation, phosphorylation and
citrullination modifications. With up to 4 modifications
per peptide, 384 different combinations are included.

 Learn More 
MODified Histone Peptide Array

Next-Gen Sequencing of

Methylated DNA

Active Motif's MeDIP and MethylCollector™ Ultra Kits
specifically enrich for methylated DNA fragments that are
ideal for use in MeDIP-Seq and MIRA-Seq.

 Learn More 
Next-Gen of Enriched Methylated DNA

Monoclonal antibodies from Active Motif

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New Products for Epigenetics Research

  • Perform ChIP Without Protein-specific Antibodies

    The Tag-ChIP-IT® Kit makes it easy to perform ChIP with transcription factor sequence variants, mutations or truncations by eliminating the need for protein-specific antibodies. Simply clone your protein sequence with Active Motif's unique AM-tag, specifically designed for use in ChIP, transfect your cells and perform ChIP using an antibody directed against the AM-tag. More >

  • Genome-wide Analysis of 5-Hydroxymethylcytosine (5-hmC)

    Active Motif's Hydroxymethyl Collector™–Seq Kit offers a highly sensitive and specific assay for genome-wide analysis of 5-hydroxymethylcytosine. The assay utilizes our proprietary method to label each 5-hmC residue with a modified glucose for specific enrichment. Enriched DNA is ready for use in Next-Generation Sequencing to determine the localization of 5-hmC within the sample. More >

  • Quantify H3K27ac Levels With Our New Histone ELISA

    Histone H3K27 acetylation is a mark of active enhancers. Active Motif offers the first commercially available ELISA to screen for changes in H3K27ac and to quantify the amount of Histone H3 lysine 27 acetylation using our recombinant histone standard. Click to learn more about the Histone H3 acetyl Lys27 ELISA. More >

  • Reduce the Effects of Technical Variation In Your ChIP

    Active Motif has developed a ChIP Normalization Strategy that can be used to normalize ChIP qPCR and ChIP-Seq data to reduce the effects of technical variation and sample processing bias. This normalization strategy can be applied across samples and antibodies. More >

  • Study the Regulation of lncRNA Promoters

    LightSwitch™ lncRNA Promoter Reporter Vectors make it fast and easy to study the unique regulation of lncRNA promoters using Active Motif’s popular LightSwitch Luciferase Assay System. Each sequence-verified, transfection-ready reporter construct contains the promoter region of a lncRNA gene cloned from the human genome, so you can begin your assays immediately. More >

  • RIME Service: Identify Protein Interactions by Mass Spectrometry

    Adding to our growing list of Epigenetic Services offerings, Active Motif now offers RIME (Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins), which allows for the identification of transcriptional co-factors and chromatin associated proteins. More >

  • Low Cell Number ChIP-Sequencing Services

    New advancements in our ChIP-Seq protocols and pipeline have reduced ChIP-Seq starting material requirements by 1,000 fold! In large contrast to the 10,000,000 cell requirement needed to perform standard ChIP-Seq, Active Motif now offers low cell number ChIP-Seq services, that allow you to perform ChIP-Seq with as few as 10,000 cells. More >

  • ChIP-Seq Validated Antibodies for Pluripotent Stem Cell Markers

    Adding to our growing collection of ChIP-Seq validated antibodies, Active Motif now offers ChIP-Seq validated antibodies specific for Oct4, Sox2, Nanog, KLF5 and TCF7L1/TCF3 stem cell pluripotency factors. More >

  • New Recombinant Nucleosomes for use in Enzymatic Assays and Chromatin Assembly

    Active Motif's Recombinant Nucleosomes offer a more biologically relevant choice of substrate when compared to histone proteins alone or synthetic peptides for use in drug discovery screening, analyzing enzyme kinetics or monitoring changes in histone modifications. Each recombinant nucleosome is comprised of octamers of unmodified core histone proteins (H2A, H2B, H3 and H4) bound by DNA. Biotin-tagged Nucleosomes are also available for pull-down or binding assays. More >

  • Validate the Specific mRNA Targets of an miRNA Using Ago IP

    The targeting of a particular microRNA (miRNA) molecule to a specific mRNA is mediated through the formation of an RNA Induced Silencing Complex (RISC). The key components in all RISCs are an Argonaute protein (Ago) and the miRNA. Active Motif’s miRNA Target IP Kit makes possible efficient immunoprecipitation of miRNA/mRNA complexes using a pan-Ago antibody and protein G-coupled magnetic beads, so you can validate physical interactions of a miRNA with endogenous mRNA transcripts. More >

  • New ChIP-Exonuclease Kit for High-Resolution ChIP Analysis

    ChIP-exo provides a high-resolution method for mapping transcription factor binding sites genome-wide. It utilizes exonuclease-mediated digestion of DNA during the immunoprecipitation protocol to remove non-cross-linked DNA and increase binding site resolution to within 20-95 bp reads. This makes ChIP-exo ideal for mapping transcription factor binding sites, performing discovery-based studies or evaluation of SNP or mutation effects. More >

  • ChIP-Bisulfite-Sequencing for Methylation Profiles of your ChIP DNA

    ChIP-Bis-Seq enables direct interrogation of both chromatin and DNA methylation profiles on the same sample. Evaluate allele-specific difference, or gene regulatory effects with reduced sequencing costs by focusing on specific genomic regions. More >

  • Easily Isolate Mononucleosomes or Oligonucleosomes

    Easily isolate mononucleosomes or oligonucleosomes from your samples using Active Motif's new Nucleosome Preparation Kit. Nucleosomes offer a more physiologically relevant substrate for histone post-translational studies, enzyme kinetic analysis and inhibitor screening studies. More >

  • Global DNA Methylation Assay to Quantify 5-mC Levels in DNA

    Active Motif's new Global DNA Methylation Assay – LINE-1 Kit is a simple, easy-to-use ELISA based assay for high throughput quantitation of global 5-methylcytosine (5-mC) levels, but is unique in that it utilizes LINE-1 methylation as a surrogate for measuring global DNA methylation. This format improves specificity of capture and sensitivity, enabling quantitation of as low as 0.5% methylation and detection of methylation from as little as 10 ng of input DNA. More >

  • Cas9 Antibody for Use in CRISPR/Cas9 Gene Editing

    Active Motif now offers a Cas9 monoclonal antibody validated for use in immunoprecipitation, immunofluorescence and Western blot experiments. While Cas9 is a component of the CRISPR/Cas prokaryotic immune system that provides protection against mobile genetic elements (viruses, transposable elements, plasmids etc.), CRISPR/Cas is used by scientists as a useful tool to insert or delete sequences at any specific location in a cell's genome. This antibody can be used to verify expression of Cas9. More >

  • PBMC ChIP Kit Offers a Complete Solution for ChIP from B and T Cells

    PBMCs (peripheral blood mononuclear cells), like other primary cells, are notoriously difficult to lyse which presents a challenge for chromatin preparation for ChIP. Active Motif's new ChIP-IT® PBMC Kit is the first commercial kit available that offers a complete solution for the ChIP assay workflow by providing reagents and protocols for chromatin preparation, ChIP and DNA purification that have been specifically formulated to work with PBMCs such as lymphocytes (T cells, B cells and NK cells) and monocytes. More >

  • Rapid Bisulfite Conversion Kit for Single-nucleotide DNA Methylation Analysis, Including Positive Control Primers for Validation

    Active Motif's Bisulfite Conversion Kit is a rapid 1.5 hour conversion protocol to convert unmethylated cytosines into uracil for single-nucleotide DNA methylation analysis. More >

  • Post-transcriptional Regulation Mediated by miRNA-3´UTR Interactions

    While gene regulation studies have historically focused on events that influence transcription, post-transcriptional regulation plays an equally important role. The binding of miRNAs to the 3´UTRs of transcribed mRNAs affect both the transcript’s stability and rate of translation. To study miRNA-3´UTR interactions, Active Motif offers over 12,000 LightSwitch™ 3´UTR Reporter constructs, as well as over 900 synthetic miRNA targets, and 1000 miRNA mimics & inhibitors. More >

  • Obtain ChIP-Seq Data from FFPE Tissue Samples

    Active Motif's new ChIP-IT® FFPE Chromatin Preparation Kit and ChIP-IT® FFPE Kit are the first kits available to perform ChIP analysis of formalin-fixed paraffin-embedded (FFPE) tissue material. Due to the limited size and harsh fixation conditions used to prepare FFPE slides and tissue blocks, these samples have previously not been useful in ChIP. Active Motif's new assays are the only kits with the sensitivity required to obtain ChIP-Seq quality DNA from FFPE tissues. More >

  • New Luminex® Multiplex Assay to Screen Histone PTMs

    Active Motif has partnered with Luminex® to develop a 96-well plate-based multiplex assay for the analysis of histone PTMs. The Histone PTM Multiplex Kit enables simultaneous analysis of up to 13 different Histone H3 modifications within the same well using only nanogram quantities of sample. More >

  • More Recombinant Methyltransferases and Demethylases to Choose From

    Active Motif has recently expanded its offering of high-quality Recombinant Histone Methyltransferases (HMTs) and Histone Demethylases (HDMs), including newly added PRC2 complexes (including complexes with EZH2 point mutations), and additional Jumonji, arginine methyltransferases and arginine demethylases. The enzymes are suitable for use in studies of enzyme kinetics, profiling and screening of inhibitors, and drug discovery and development. More >

  • Over 30,000 Pre-Cloned Regulatory Elements for Reporter Assays

    The LightSwitch™ Luciferase Assay System includes over 18,000 promoters and 12,000 3´UTRs cloned from the human genome and available as transfection-ready LightSwitch luciferase reporter vectors. Other LightSwitch products include Pathway Reporter Stable Cell Lines, Synthetic miRNA Targets, and miRNA Mimics and Inhibitors. More >

  • MODified™ Protein Domain Binding Kit to Assay Specificity of Protein Reading Domains

    The MODified™ Protein Domain Binding Kit is designed to be used in combination with Active Motif's MODified Histone Peptide Array to screen His-tagged protein domains for interaction with histone and their post-translational modifications. The kit contains optimized buffers and reagents for analysis of protein domain binding specificity along with HRP-conjugated antibodies and ECL reagents chemiluminescent detection. For added convenience, a positive control tudor domain protein is also provided.More >

  • Novel Stem Cell Dye for the Identification of Live Stem Cells

    Stem Cell CDy1 Dye is designed for selective staining of live stem cells. The CDy1 dye can be used for identification and isolation of various cell types including ESCs, iPSCs, neural stem cells and cancer stem cells by fluorescence imaging or FACS analysis. More >

  • ChIP-IT® ChIP-Seq Kit for Whole-genome ChIP Analysis

    Active Motif now offers a complete workflow for ChIP-Seq experiments with our new ChIP-IT® ChIP-Seq Kit. The Kit provides proven reagents and protocols for successful ChIP-Seq results. More >

  • High Sensitivity ChIP Kit

    Obtain high quality ChIP DNA when working with low abundance transcription factor targets, antibodies with sub-optimal binding affinities or when using as few as 1,000 cells per IP with Active Motif's ChIP-IT® High Sensitivity Kit. More >

  • Ready-to-Use Protein G Agarose Columns for ChIP

    Active Motif's Protein G Agarose Columns contain pre-washed Protein G agarose loaded into columns and ready to use for ChIP. Simply add the ChIP reaction to the column and go. Wash steps are also performed in the column which makes the process faster and also ensures complete sample recovery for better reproducibility. More >

  • Recombinant Bromodomain Proteins

    Active Motif has just introduced a large number of high-quality Recombinant Bromodomain proteins for use in studies of bromodomain function in transcriptional regulation and chromatin remodeling. More >

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