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Chromeo™ Dyes – Chromeo 488, Chromeo 494, Chromeo 505, Chromeo 546 and Chromeo 642 – are bright fluorescent labels that replace Alexa Fluor*, DyLight* or Cy*-dyes. They exhibit superior luminescence properties, broad Stokes shifts, stability towards photobleaching and pH, and low cell toxicity. In addition, they are compatible with most excitation sources including diode lasers, LEDs, tungsten lamps and xenon arc lamps. For your labeling convenience, Chromeo Dyes are offered in a variety of chemistries, as well as conjugated to secondary antibodies.
Chromeo™ Dye advantages
- High intensity
- Photostability – the combination with MAX Stain™ reagents provides optimal fluorescent stability for multiple exposures and increased exposure time
- Room temperature incubations
- pH insensitive
- Low background
- Easy to use – no harsh chemicals required for conjugation
Reactive fluorescent Chromeo Dyes serve as bright labels for antibodies and other biomolecules, enabling detection in immunofluorescence, high content screening, ELISA, FRET applications or flow cytometry. For your convenience, Chromeo Dyes are also available as part of our easy-to-use Fluorescent Antibody Labeling Kits, and are offered as optimized Fluorescent Secondary Antibody Conjugates for enhanced detection.
| Dye | Absorption | Emission | Spectra | ε L/(mol-cm) | Stokes shift |
|---|---|---|---|---|---|
| Chromeo™ 488 | 488 nm | 517 nm |  |
73,000 | 29 nm |
| Chromeo™ 494 | 494 nm | 628 nm |  |
55,000 | 124 nm |
| Chromeo™ 505 | 505 nm | 526 nm |  |
70,000 | 21 nm |
| Chromeo™ 546 | 545 nm | 561 nm |  |
98,800 | 16 nm |
| Chromeo™ 642 | 642 nm | 660 nm |  |
180,000 | 18 nm |
| Table 1: Properties of Chromeo Dyes. | |||||
To ensure that you get the best results possible, we highly recommend that you use Chromeo™ Dyes together with our MAX Stain™ Immunofluorescence Tools, as those components have been formulated to optimize the performance of the Chromeo™ Dyes.
| Name | Format | Cat. No. | Price | |
|---|---|---|---|---|
| Chromeo™ 488 Carboxylic Acid | 1 mg | 15510 | $95 |
 |
| 5 mg | 16510 | $380 |
|
|
| Chromeo™ 488 NHS-Ester | 1 mg | 15511 | $195 |
|
| 5 mg | 16511 | $775 |
|
|
| Chromeo™ 488 Biotin | 1 mg | 15512 | $165 |
|
| 5 mg | 16512 | $675 |
|
|
| Chromeo™ 488 Streptavidin | 1 mg | 15513 | $225 |
|
| 5 mg | 16513 | $895 |
|
|
| Chromeo™ 494 Carboxylic Acid | 1 mg | 15110 | $95 |
|
| 5 mg | 16110 | $380 |
|
|
| Chromeo™ 494 NHS-Ester | 1 mg | 15111 | $195 |
|
| 5 mg | 16111 | $775 |
|
|
| Chromeo™ 494 Biotin | 1 mg | 15112 | $165 |
|
| 5 mg | 16112 | $675 |
|
|
| Chromeo™ 494 Streptavidin | 1 mg | 15113 | $225 |
|
| 5 mg | 16113 | $895 |
|
|
| Chromeo™ 505 Carboxylic Acid | 1 mg | 15610 | $95 |
|
| 5 mg | 16610 | $380 |
|
|
| Chromeo™ 505 NHS-Ester | 1 mg | 15611 | $195 |
|
| 5 mg | 16611 | $775 |
|
|
| Chromeo™ 546 Carboxylic Acid | 1 mg | 15210 | $95 |
|
| 5 mg | 16210 | $380 |
|
|
| Chromeo™ 546 NHS-Ester | 1 mg | 15211 | $195 |
|
| 5 mg | 16211 | $775 |
|
|
| Chromeo™ 546 Biotin | 1 mg | 15212 | $165 |
|
| 5 mg | 16212 | $675 |
|
|
| Chromeo™ 546 Streptavidin | 1 mg | 15213 | $225 |
|
| 5 mg | 16213 | $895 |
|
|
| Chromeo™ 642 Carboxylic Acid | 1 mg | 15310 | $95 |
|
| 5 mg | 16310 | $380 |
|
|
| Chromeo™ 642 NHS-Ester | 1 mg | 15311 | $195 |
|
| 5 mg | 16311 | $775 |
|
|
| Chromeo™ 642 Biotin | 1 mg | 15312 | $165 |
|
| 5 mg | 16312 | $675 |
|
|
| Chromeo™ 642 Streptavidin | 1 mg | 15313 | $225 |
|
| 5 mg | 16313 | $895 |
|
