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Fluorescence

Fluorescent Chromeo™ Dyes

effective fluorescent labels for bioanalysis

Chromeo™ Dyes – Chromeo 488, Chromeo 494, Chromeo 505, Chromeo 546 and Chromeo 642 – are bright fluorescent labels that replace Alexa Fluor*, DyLight* or Cy*-dyes. They exhibit superior luminescence properties, broad Stokes shifts, stability towards photobleaching and pH, and low cell toxicity. In addition, they are compatible with most excitation sources including diode lasers, LEDs, tungsten lamps and xenon arc lamps. For your labeling convenience, Chromeo Dyes are offered in a variety of chemistries, as well as conjugated to secondary antibodies.

Chromeo™ Dye advantages

  • High intensity
  • Photostability – the combination with MAX Stain™ reagents provides optimal fluorescent stability for multiple exposures and increased exposure time
  • Room temperature incubations
  • pH insensitive
  • Low background
  • Easy to use – no harsh chemicals required for conjugation

Reactive fluorescent Chromeo Dyes serve as bright labels for antibodies and other biomolecules, enabling detection in immunofluorescence, high content screening, ELISA, FRET applications or flow cytometry. For your convenience, Chromeo Dyes are also available as part of our easy-to-use Fluorescent Antibody Labeling Kits, and are offered as optimized Fluorescent Secondary Antibody Conjugates for enhanced detection.

To receive more detailed information and application data about individual Chromeo dyes, simply click on the name of the Chromeo dye of your choice in the table below. To see a larger spectra image, click on the spectrum of your choice.

Dye Absorption Emission Spectra ε L/(mol-cm) Stokes shift
Chromeo™ 488 488 nm 517 nm Chromeo 488 Fluorescent Dye Spectra 73,000 29 nm
Chromeo™ 494 494 nm 628 nm Chromeo 494 Fluorescent Dye Spectra 55,000 134 nm
Chromeo™ 505 505 nm 526 nm Chromeo 505 Fluorescent Dye Spectra 70,000 21 nm
Chromeo™ 546 545 nm 561 nm Chromeo 546 Fluorescent Dye Spectra 98,800 16 nm
Chromeo™ 642 642 nm 660 nm Chromeo 642 Fluorescent Dye Spectra 180,000 18 nm
Table 1: Properties of Chromeo Dyes.

 

To ensure that you get the best results possible, we highly recommend that you use Chromeo™ Dyes together with our MAX Stain™ Immunofluorescence Tools, as those components have been formulated to optimize the performance of the Chromeo™ Dyes.

 
Name Format Cat No. Price  
Chromeo™ 488 Alkyne 1 mg 15290 $240 Buy Now
5 x 1 mg 15291 $925 Buy Now
Chromeo™ 488 Carboxylic Acid 1 mg 15510 $115 Buy Now
5 x 1 mg 16510 $445 Buy Now
Chromeo™ 488 NHS-Ester 1 mg 15511 $230 Buy Now
5 x 1 mg 16511 $865 Buy Now
Chromeo™ 488 Streptavidin 1 mg 15513 $210 Buy Now
5 x 1 mg 16513 $825 Buy Now
Chromeo™ 494 Azide 1 mg 15282 $240 Buy Now
5 x 1 mg 15283 $925 Buy Now
Chromeo™ 494 Alkyne 1 mg 15292 $240 Buy Now
5 x 1 mg 15293 $925 Buy Now
Chromeo™ 494 Carboxylic Acid 1 mg 15110 $115 Buy Now
5 x 1 mg 16110 $445 Buy Now
Chromeo™ 494 NHS-Ester 1 mg 15111 $230 Buy Now
5 x 1 mg 16111 $865 Buy Now
Chromeo™ 494 Streptavidin 1 mg 15113 $210 Buy Now
5 x 1 mg 16113 $825 Buy Now
Chromeo™ 505 Carboxylic Acid 1 mg 15610 $115 Buy Now
5 x 1 mg 16610 $445 Buy Now
Chromeo™ 505 NHS-Ester 1 mg 15611 $230 Buy Now
5 x 1 mg 16611 $865 Buy Now
Chromeo™ 546 Azide 1 mg 15284 $240 Buy Now
5 x 1 mg 15285 $925 Buy Now
Chromeo™ 546 Alkyne 1 mg 15294 $240 Buy Now
5 x 1 mg 15295 $925 Buy Now
Chromeo™ 546 Carboxylic Acid 1 mg 15210 $115 Buy Now
5 x 1 mg 16210 $445 Buy Now
Chromeo™ 546 NHS-Ester 1 mg 15211 $230 Buy Now
5 x 1 mg 16211 $865 Buy Now
Chromeo™ 546 Streptavidin 1 mg 15213 $210 Buy Now
5 x 1 mg 16213 $825 Buy Now
Chromeo™ 642 Azide 1 mg 15288 $240 Buy Now
5 x 1 mg 15289 $925 Buy Now
Chromeo™ 642 Alkyne 1 mg 15296 $240 Buy Now
5 x 1 mg 15297 $925 Buy Now
Chromeo™ 642 Carboxylic Acid 1 mg 15310 $115 Buy Now
5 x 1 mg 16310 $445 Buy Now
Chromeo™ 642 NHS-Ester 1 mg 15311 $230 Buy Now
5 x 1 mg 16311 $865 Buy Now
Chromeo™ 642 Streptavidin 1 mg 15313 $210 Buy Now
5 x 1 mg 16313 $825 Buy Now