Low Cell Number ChIP-Seq
Genome-wide binding profiles from as few as 50,000 cells
Standard ChIP-Seq protocols require at least 10,000,000 cells to perform ChIP-Seq, a number that is unattainable for many primary cell types. As a result, for some cell types, one of the major obstacles preventing researchers from generating quality ChIP-Seq data is the limited cell numbers available for those cell systems. Until now.
Active Motif’s Epigenetic Services group has made significant improvements to our ChIP-Seq protocols and pipeline, and as a result, we have reduced starting material requirements by 1,000 fold!
See for yourself, browse some of our complete data sets:
Ideal for the analysis of:
- Live FACS sorted cells.
- Samples isolated from magnetic-activated cell sorting.
- Primary tissues or cells such as pancreatic Beta cells.
- Limited patient samples.
- Small human biopsies.
- 50,000 cells for histone modifications.
- 100,000 cells for transcription factors and histone modifying enzymes.
The Low Cell Number ChIP-Seq Service includes:
Customers submit cell pellets or small frozen tissues, then we:
- Fix samples, prepare chromatin and sonicate.
- Perform ChIP with a ChIP-validated antibody.
- Construct ChIP-Seq libraries.
- Perform Next-Gen sequencing.
- Analyze the data and deliver it to the customer.
Figure 1: Comparison of ChIP-Seq data generated with 50,000 and 10,000 cells
Figure 2: Comparison of ChIP-Seq data generated with 10,000 cells
Figure 3: Comparison of ChIP-Seq data generated with 50,000 and 10,000 cells