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Active Motif's Chromeo™ Antibody Labeling Kits enable you to label your antibodies and proteins with the superior fluorescence of our Chromeo Dyes. Convenient kits for labeling with the Chromeo 488, 494, 546 and 642 Dyes are available. Chromeo conjugates will exhibit a bright fluorescence and additional superior luminescence properties, like broad Stokes shifts, stability towards photobleaching and pH, and low cell toxicity.
Figure 1: Tubulin staining in HeLa cells.
HeLa cells were stained with alpha Tubulin mouse mAb (Clone 5-B-1-2) and Chromeo 488 Goat anti-mouse IgG (Catalog No. 15031), Chromeo 546 Goat anti-mouse IgG (Catalog No. 15033) and Chromeo 642 Goat anti-mouse IgG (Catalog No. 15034). The nuclei in the 488 and 642 images have been counterstained with DAPI.
To ensure that you get the best results possible, we highly recommend that you use Chromeo Dyes together with our MAX Stain™ Immunofluorescence Tools, as those components have been formulated to optimize the performance of the Chromeo Dyes.
| Name | Format | Cat. No. | Price | |
|---|---|---|---|---|
| Chromeo™ 488 Antibody Labeling Kit | 1 kit | 15090 | $295 |
 |
| Chromeo™ 494 Antibody Labeling Kit | 1 kit | 15091 | $295 |
|
| Chromeo™ 546 Antibody Labeling Kit | 1 kit | 15092 | $295 |
|
| Chromeo™ 642 Antibody Labeling Kit | 1 kit | 15093 | $295 |
|
Chromeo Antibody Labeling Kits provide the Chromeo Dye of your choice lyophilized in convenient one-time-use amounts with purification columns and reagents for three labeling reactions. Each kit provides sufficient activated amine-reactive Chromeo Dye and reagents for 3 separate reactions that will label 1 mg of antibody each.
Figure 1: Lamin staining in fixed HeLa cells.
A mouse secondary antibody that was labeled using the Chromeo™ 488 Antibody Labeling Kit was used to detect a primary Lamin A antibody in HeLa cells that had been fixed and permeabilized using formaldehyde.
Chromeo Antibody Labeling Kit advantages
- Easy – just mix dye and antibody
- Quick – labeled antibody ready in 1 hour
- Affordable – costs much less than custom orders
- Optimized – rely on Active Motif Chromeon expertise
Chromeo™ Dyes – Chromeo 488, Chromeo 494, Chromeo 546 and Chromeo 642 – are bright fluorescent labels that replace Alexa Fluor*, DyLight* or Cy*-dyes. They are compatible with most excitation sources including diode lasers, LEDs, tungsten lamps and xenon arc lamps.
Chromeo™ Dye advantages
- High intensity
- Photostability – the combination with MAX Stain™ reagents provides optimal fluorescent stability for multiple exposures and increased exposure time
- Room temperature incubations
- pH insensitive
- Low background
- Easy to use – no harsh chemicals required for conjugation
Fluorescent Chromeo Dyes serve as bright labels for antibodies and other biomolecules, enabling detection in immunofluorescence, high content screening, ELISA and FRET applications, as well as in flow cytometry.
| Dye | Absorption | Emission | Spectra | ε L/(mol-cm) | Stokes shift |
|---|---|---|---|---|---|
| Chromeo™ 488 | 488 nm | 517 nm |  |
73,000 | 29 nm |
| Chromeo™ 494 | 494 nm | 628 nm |  |
55,000 | 124 nm |
| Chromeo™ 546 | 545 nm | 561 nm |  |
98,800 | 16 nm |
| Chromeo™ 642 | 642 nm | 660 nm |  |
180,000 | 18 nm |
| Table 1: Properties of Chromeo Dyes. | |||||
To recieve more detailed information and application data about individual Chromeo dyes, simply "click" on the name or on the spectrum of your Chromeo dye of your choice in the table above.
Contents & Storage
3 vials of Chromeo Dye, 3 purification columns, Coupling Buffer, Elution Buffer and DMF. Chromeo Dyes should be stored at -20°C and all other components should be stored at 4°C. All reagents are guaranteed stable for 6 months when stored properly.
The 1 ml vial of MAXfluor™ Mounting Medium, which is delivered with the Chromeo 488 Antibody Labeling Kit, should be stored at 4°C in the dark.
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