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Active Motif's fluorescent secondary conjugates offer an improved method for detection of your primary antibodies. These secondary antibodies are conjugated a number of high-quality dyes, including the Chromeo™ line of fluorescent dyes, giving you a variety of choices for fluorescent detection. To conjugate the Chromeo Dyes to primary antibodies and other proteins, we offer our complete, optimized antibody/protein labeling kits.
The ATTO dye conjugates have been maximally cross-adsorbed against IgG's of a variety of species to eliminate background caused by non-specific binding. The ATTO STED dyes have been certified by Leica Microsystems for Stimulated Emission Depletion (STED) microscopy.
Fluorescent Secondary advantages
- Unrivaled fluorescent intensity
- Low background
- Limited photobleaching
Figure 1: Tubulin staining with Chromeo 488 secondary antibody.
U2OS cells stained with propidium iodide were incubated with rabbit anti-tubulin antibody. Chromeo 488 Goat anti-Rabbit IgG secondary antibody was then used at 1:1000.
Figure 2: EGFR staining with Chromeo 546 secondary antibody.
The Chromeo 546 Goat anti-Mouse IgG secondary antibody was used to stain EGFR in an urothel cell line. This data was generously provided by Dr. Brockhoff, Institut for Pathology, University Regensburg, Germany.
