Capillary Electrophoresis (CE) Dye Kits
capillary electrophoresis made easy
The CE Dye Kits provide a convenient alternative for the fluorescent labeling of protein samples prior to capillary electrophoresis. Capillary electrophoresis (CE) has proven to be a useful tool in modern cell biology applications because it enables the rapid and high-resolution separation of proteins in a microsample format. These properties have made CE especially useful when performing single-cell protein analyses. However, traditional CE performed using UV absorbance can suffer from a lack of sensitivity due to the short path length across the capillary.
The CE Dye advantage
- User-friendly, non-toxic labeling
- Large Stokes shift for reduced background
- Fast and simple procedure
- Sensitive protein detection
CE Dye Kits provide a highly sensitive, convenient solution for capillary electrophoresis (CE). Upon binding to protein, CE Dyes undergo a large Stokes shift as well as a 50-fold increase in quantum yield. The result is sensitive detection with minimal background.
CE Dyes overcome a large limitation associated with derivatization-based methods for labeling proteins because the positive charge of the amine group is maintained even after dye conjugation (Figure 1). This means that every protein labeled with CE Dye maintains its native charge, so you get single peak protein detection. With CE Dyes, there is no band broadening or need to perform adjusted ionic character calculations.
For your convenience, two CE Dye Kits are offered. CE Dye 503 requires excitation at 503 nm, while CE Dye 540 requires excitation at 540 nm; both share a common emmision maximum at 600 nm.
Figure 1: Labeling with CE Dyes maintains each protein's native charge.
Figure 2: Color shift caused by dye conjugation.
CE Dyes have been successfully used as a stain in capillary electrophoresis. The detection limit in the picomolar range, the fast reaction time and the preservation of the protein’s native charge make our CE Dyes an interesting alternative to commonly used detection techniques.
- “Determination of picomolar concentrations of protein using novel amino reactive chameleon labels and capillary electrophoresis laser-induced fluorescence detection” by Craig et al (2005) Electrophoresis 26(11):2208-2213.
- “Reaction of organic reagents with proteins III. Spectroscopic and electrophoretic behavior of proteins labeled with Chromeo P503” by Turner et al (2008) J. of Chromatography 1194(2):253-256.
- “Determination of biogenic amines by capillary electrophoresis using a chameleon type of fluorescent stain” by Steiner et al (2009) Microchimica Acta 167 (3-4):259-266.
- “Polymer microchip CE of proteins either off- or on-chip labeled with chameleon dye for simplified analysis” by Yu et al (2009) Electrophoresis 30(24):4230-4236.
- “Protein labeling Enhances Aptamer Selection by Methods of Capillary Electrophoresis” by de Jong et al (2011) Anal. Chem. 83:6330-6335.
- “Preasure-Based Approach for the Analysis of Protein Adsorption in Capillary Electrophoresis; by de Jong et al (2012) Anal. Chem. 84:453-458.
- “On-capillary fluorescent labeling and CE-LIF analysis of glycoforms of intact prostate-specific antigen; by Garrido-Medina et al (2013) Electophoresis 34(16):2295-2302.
Contents & Storage
Each CE Dye Kit supplies sufficient CE Dye and Reaction Buffer for performing 200-300 labeling reactions. All reagents can be stored at 4°C and are guaranteed stable for 6 months when stored properly.