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The CE Dye Kits provide a convenient alternative for the fluorescent labeling of protein samples prior to capillary electrophoresis. Capillary electrophoresis (CE) has proven to be a useful tool in modern cell biology applications because it enables the rapid and high-resolution separation of proteins in a microsample format. These properties have made CE especially useful when performing single-cell protein analyses. However, traditional CE performed using UV absorbance can suffer from a lack of sensitivity due to the short path length across the capillary.
The CE Dye advantage
- User-friendly, non-toxic labeling
- Large Stokes shift for reduced background
- Fast and simple procedure
- Sensitive protein detection
CE Dye Kits provide a highly sensitive, convenient solution for capillary electrophoresis (CE). Upon binding to protein, CE Dyes undergo a large Stokes shift as well as a 50-fold increase in quantum yield. The result is sensitive detection with minimal background.
CE Dyes overcome a large limitation associated with derivatization-based methods for labeling proteins because the positive charge of the amine group is maintained even after dye conjugation (Figure 1). This means that every protein labeled with CE Dye maintains its native charge, so you get single peak protein detection. With CE Dyes, there is no band broadening or need to perform adjusted ionic character calculations.
For your convenience, two CE Dye Kits are offered. CE Dye 503 requires excitation at 503 nm, while CE Dye 540 requires excitation at 540 nm; both share a common emmision maximum at 600 nm.
Figure 1: Labeling with CE Dyes maintains each protein's native charge.
Chemical equation depicting the chemistry of CE Dye labeling, which demonstrates the preservation of the positive charge of the lysine residue of a protein. R stands for the respective chromogenic/fluorogenic group.
Figure 2: Color shift caused by dye conjugation.
CE Dyes are blue while in their free state. After a short conjugation reaction, the dyes' color undergoes a large Stokes shift and becomes red.
