Histone PTM Quantitation Service
quantify multiple histone post-translational modifications
Active Motif's Histone PTM Quantitation Service gives you access to ground-breaking technology that enables high-throughput multiplexed screening of histone modifications. The service uses Luminex® xMAP® magnetic bead-based multiplexing technology to simultaneously measure multiple histone modification targets in a single reaction. This first-of-its-kind multiplex epigenetic assay generates more data with less sample than traditional western blot or ELISA methods. To learn more about our Histone PTM Multiplex kits and reagents, click here.
Technology covered under U.S. Patent No. 8367334 b2.
Available modifications for multiplex analysis
- Minimal cell requirement of 250,000 cells is sufficient to quantify 13 histone modifications.
- Detects ≥ 10% difference between samples.
- Controls include total H3 and samples containing known PTM changes.
Histone PTM Quantitation Service includes:
- Assay design.
- Sample preparation.
- Completion of the Luminex assay.
- Data analysis.
|Histone PTM Quantitation, 8 Sample Service||25062||Request Quote|
|Luminex Histone PTM Bead Set||25063||Request Quote|
|Histone PTM Cell Prep Protocol|
|Histone PTM Sample Submission form|
|Epigenetic Services Citations|
|Newsletter - October 2015|
Figure 1: Data from our Histone PTM Quantitation Service shows increased histone acetylation in response to SAHA-mediated HDAC inhibition
Figure 2: HDAC inhibitor (VPA) treatment increases histone acetylation with concomitant reductions in H3K27me2
Figure 3: EZH2 inhibition results in decreased H2K27me3 levels