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LavaCell™ is a small, neutral, non-toxic compound that readily diffuses into live or fixed cells and spontaneously reacts to produce bright orange fluorescence. This naturally fluorescent compound is excited by a variety of common lasers and can be multiplexed with a wide range of common blue, green and yellow-emitting probes.
| Name | Format | Cat. No. | Price | |
|---|---|---|---|---|
| LavaCell™ | 200 µg | 15004 | $235 |
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Because LavaCell is a neutral molecule that crosses cell membranes, cells require no pre-treatment or permeabilization prior to staining. LavaCell rapidly reacts with free amine groups on proteins, which produces a bright orange fluorescent signal (Figure 1). LavaCell does not stain nucleic acids nor does it adversely affect cell growth. LavaCell can be excited with common laser sources (405, 488 and 532 nm) and has a Stokes shift of over 100 nm with peak fluorescence emission at 610 nm.
Figure 1: Staining of live CHO cells with LavaCell.
LavaCell stains the plasma membrane and internal membranes, such as those of the nucleus, Golgi and ER. CHO cells were plated in 96-well plates cells and treated with 6 µM LavaCell (diluted in complete medium) for 30 minutes at 37°C. After loading, the dye containing DMEM was removed and cells were washed once in PBS. Cells were imaged in PBS using a fluorescent microscope with a 540-580 nm excitation filter and a 600-660 nm emission filter (exposure time 10 seconds, 40X objective).
LavaCell advantages
- Water soluble fluorescent cell stain
- Fluoresces on interaction with proteins; no need to wash away unbound stain
- Readily permeates into cells
- No effect on cell growth or viability
- Ideal for multiplexing with other fluorophores due to long Stokes shift
Contents and Storage
200 µg (1 mg/ml) in DMSO. Store at -20°C in the dark. Contents guaranteed 6 months when stored properly.
