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Antibodies

AbFlex™ Recombinant Antibodies

defined Abs for highly specific, reproducible performance

AbFlex™ antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. The unique advantages of the AbFlex antibody are its flexible labeling and purification options. Each AbFlex antibody contains a Sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner using our Sortag-IT Labeling Kits. Every antibody also contains a 6xHis tag, which can be used with nickel-based purification systems, and an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

AbFlex antibody with flexible labeling and purification options
AbFlex™ recombinant antibody (rAb) with flexible labeling and purification options.

 

AbFlex antibodies are specifically labeled at the end of the constant region of the heavy chain to avoid interference with antigen recognition and functionality. This is important as it ensures the labeling process maintains the integrity of the antibody so signal is not diminished as a result of non-functional antibodies. In contrast, commonly used chemical labeling methods add labels to the antibody in a random fashion. The randomness of this process has a high potential to block the antigen-binding site and render the antibody ineffective. Chemical labeling can also deposit labels on the Fc region of the antibody which has the potential to obstruct interactions with protein A.

AbFlex directed labeling versus chemical labeling
Differences in label distribution between AbFlex™ direct labeling and chemical labeling.

 

Prior to the development of recombinant antibodies, researchers relied on polyclonal and monoclonal antibodies. Polyclonal antibodies recognize multiple epitopes on the same antigen. This increases antibody affinity, but often has a higher potential for cross-reactivity. Monoclonal antibodies represent a single antibody producing B cell that binds to one unique epitope. However, monoclonal antibodies can be lost or evolve over time.

Unlike monoclonal antibodies, which can experience gene loss, mutations and cell-line drift that alter antibody performance over time and lead to variability across antibody lots, recombinant antibodies are highly consistent because they contain the same immunoglobulin heavy and light chain DNA sequences in every lot. This provides batch-to-batch reproducibility in antibody performance and eliminates the need to re-evaluate or compare different antibody lots, thereby, saving valuable time and effort on assay development.

To ensure optimal performance, each AbFlex antibody is carefully screened for specificity and sensitivity. Only heavy and light chain sequences that produce highly specific antibodies, as verified in our bead-based binding assay, are selected. Antibodies that utilize the AbFlex direct labeling approach also show greater sensitivity than their parent monoclonal antibodies across a variety of applications.

Another advantage of the AbFlex recombinant antibodies is the fact that they are produced outside of an animal system. Polyclonal antibodies rely on an animal’s immune response and epitope specificity, both of which are highly variable across animals and immunizations. Polyclonal antibodies are also hindered by having a finite supply, making it difficult to perform long-term antibody projects or reproduce data over time or across labs. With AbFlex recombinant antibodies, the full-length IgG is expressed in a mammalian system to ensure a consistent, stable antibody supply. Additionally, any ethical or animal welfare concerns associated with antibody production and maintenance are avoided.

Advantages of AbFlex™ Recombinant Antibodies (rAbs)

  • Flexible labeling & purification options
  • Consistent performance across lots
  • Highly specific and sensitive antibodies
  • Reliable, long-term supply
  • Animal-free production

The following list contains Active Motif's AbFlex™ recombinant antibodies (rAbs). For information about site-specific labeling of the AbFlex antibodies, please visit our collection of Sortag-IT™ Labeling Kits.

 
Name Isotype Reactivity Appl. Format Cat No. Price  
AbFlex™ 5-methylcytosine antibody (rAb) IgG2a Other (Not Species Specific) DB 100 µg 91187 $375 Buy Now
10 µg 91188 $75 Buy Now
AbFlex™ Cas9 antibody (rAb) IgG2a Human ELISA, WB 100 µg 91123 $375 Buy Now
10 µg 91124 $75 Buy Now
AbFlex™ EED antibody (rAb) IgG2a Human ChIP, ChIP-Seq, ELISA 100 µg 91135 $375 Buy Now
10 µg 91136 $75 Buy Now
AbFlex™ HIF-1 alpha antibody (rAb) IgG2a Bovine, Human, Mouse, Rat ELISA 100 µg 91139 $375 Buy Now
10 µg 91140 $75 Buy Now
AbFlex™ His Tag antibody (rAb) IgG2a Other (Not Species Specific) WB 100 µg 91183 $375 Buy Now
10 µg 91184 $75 Buy Now
AbFlex™ AM-Tag antibody (rAb) IgG2a Drosophila ChIP, ELISA, WB 100 µg 91111 $375 Buy Now
10 µg 91112 $75 Buy Now
AbFlex™ AM-Tag antibody (rAb) (biotin) IgG2a Drosophila ChIP, ELISA, WB 100 µg 91109 $405 Buy Now
10 µg 91110 $85 Buy Now
AbFlex™ Histone H3K9me0 Antibody (rAb) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91155 $375 Buy Now
10 µg 91156 $75 Buy Now
AbFlex™ Histone H3K9ac antibody (rAb) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91103 $375 Buy Now
10 µg 91104 $75 Buy Now
AbFlex™ Histone H3K9ac antibody (rAb) (biotin) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91101 $405 Buy Now
10 µg 91102 $85 Buy Now
AbFlex™ Histone H3K56ac antibody (rAb) IgG2a Human, Other (Wide Range) ChIP-Seq, ELISA 100 µg 91127 $375 Buy Now
10 µg 91128 $75 Buy Now
AbFlex™ Histone H4K20me3 antibody (rAb) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91107 $375 Buy Now
10 µg 91108 $75 Buy Now
AbFlex™ Histone H4K20me3 antibody (rAb) (biotin) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91105 $405 Buy Now
10 µg 91106 $85 Buy Now
AbFlex™ JMJD2A antibody (rAb) IgG2a Human ELISA, WB 100 µg 91143 $375 Buy Now
10 µg 91144 $75 Buy Now
AbFlex™ Lamin A/C antibody (rAb) IgG2a Human WB 100 µg 91189 $375 Buy Now
10 µg 91190 $75 Buy Now
AbFlex™ RNA Pol II antibody (rAb) IgG2a Human ChIP-Seq, IF, WB 100 µg 91151 $375 Buy Now
10 µg 91152 $75 Buy Now
AbFlex™ RNA pol II CTD phospho Ser2 antibody (rAb) IgG2a Human ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91115 $375 Buy Now
10 µg 91116 $75 Buy Now
AbFlex™ RNA pol II CTD phospho Ser5 antibody (rAb) IgG2a Human ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91119 $375 Buy Now
10 µg 91120 $75 Buy Now
AbFlex™ RNA pol II CTD phospho Ser5 antibody (rAb) (biotin) IgG2a Human ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91117 $405 Buy Now
10 µg 91118 $85 Buy Now
AbFlex™ TET1 antibody (rAb) IgG2a Mouse WB 100 µg 91171 $375 Buy Now
10 µg 91172 $75 Buy Now