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LigandLink™ Universal Labeling* enables specific in vivo, fluorescent labeling of your protein of interest. Simply express your protein as a LigandLink fusion, then add the LigandLink Label of your choice to the medium. To change the properties of the tag, just add a different LigandLink Label. This means that you only need to clone your gene once, but can label it with a variety of tags, depending on the needs of your experiment.
*Patent pending.
Why use LigandLink™?
- Label your protein in living cells – LigandLink Labels are cell permeable
- Spend less time cloning – rather than using multiple vectors, clone only once; obtain different functionalities using different LigandLink Labels
- Small label – LigandLink Labels are unlikely to alter the characteristics of your protein of interest
LigandLink™ applications
- Protein localization & trafficking
- Protein:protein interactions
- Protein capture
Pre-made translocation vectors
Signal transduction pathways typically involve the movement of proteins throughout the cell in response to activation by some particular stimulus. The LigandLink Universal Labeling technology is ideal for studying such translocation events because it makes it simple to label the proteins in vivo. To make it even easier, we have already cloned a number of transcription factors into the pLL-1 vector, such as NFκB p65, which are ready to transfect into the mammalian cell line of your choice.
Figure 4: The LigandLink pLL-1 vector.
User friendly vector
The LigandLink vector, pLL-1, was designed for convenience of use. It features a CMV promoter for high-level expression of eDHFR fusion proteins, with Kanamycin for selection of stable cell lines. The multiple cloning site (MCS) was designed to facilitate cloning, whatever method you use. In addition to many popular restriction sites, the MCS includes three blunt-cutting restriction enzymes toward the 3´ end, each in a different reading frame with the eDHFR gene. This makes possible a number of PCR and restriction enzyme cloning strategies.
Ideal for multi-image capture experiments
Analysis of co-repressor and co-activator interactions is an important part of understanding protein function. However, analyzing interactions across multiple proteins can be time consuming and expensive. This is because each interacting partner must either be cloned into a different FP fusion vector, or primary antibodies must be labeled with multiple dyes. To overcome this problem, LigandLink Label comes in multiple formats that can be easily interchanged, depending on the FP partner of the interacting protein of interest, which saves you time and money.
