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Ras GTPase Chemi ELISA Kit

fast, sensitive analysis of activated Ras GTPase

Active Motif's Ras GTPase Chemi ELISA Kit is the first ELISA-based kit designed to detect and quantify activated Ras GTPase. Because of its ELISA format, it is much more sensitive than pull-down/Western methods. This enables the detection of low levels of protein, while also using less sample. In addition, ELISAs are more quantitative than Westerns, and they eliminate the need to run and develop gels.

Ras GTPase Chemi ELISA advantages

More sensitive – assay uses only 25 µg of extract, which is 20-fold less than pull-down/Western methods
Better results – quantitative readouts make it easier to compare results
Less effort – no need to run gels or develop Western blots
Save time – results in less than 5 hours
Versatile – assay activated extracts from cells or tissue samples, or study recombinant Ras protein

Figure 1: Quantification of activated Ras in stimulated HeLa cells.

Increasing amounts of whole-cell extract from HeLa cells that had been stimulated with 5 ng/ml of EGF for 2 minutes were assayed using the Ras GTPase Chemi ELISA Kit.

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