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As mitosis is the most defining stage of cell growth, Active Motif's chemiluminescent and colorimetric Mitotic Assay Kits are an easy way to test the effect of various treatments on cellular division. The kits utilize the same highly specific mitosis marker antibody as the fluorescent Mitotic Index Assay, but offer an alternative colorimetric or chemiluminescent readout, enabling you to choose whichever assay format best suits for your needs.
Cell division is a complex, tightly regulated process that is marked by mitosis. Thus, accurate measurement of cells undergoing mitosis is a valuable method to characterize the effects of test compounds on cellular progression. The Mitotic Assay Kits include paclitaxel for use as a positive control as it arrests cells in late G2/M phase, which provides a reference point that can be used to compare the effect of other treatments.
Figure 1: Fold induction of mitosis in HeLa cells.
HeLa cells were seeded at 20,000 cells per well and assayed using the Colorimetric (A) and Chemiluminescent (B) Mitotic Assay Kits. For a reference mitosis population, cells were treated with 1 µM paclitaxel for 6 hours. Results are the average from eight wells.
