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Active Motif’s Nitric Oxide Quantitation Kit is faster and easier-to-use than existing methods for measuring the production of NO in biological samples. The kit employs an innovative cofactor technology that reduces the time and number of steps required. The assay is also linear over a broader range of sample concentrations, which means that you’ll have to dilute and re-assay your samples far less frequently than with other methods (see Data tab). The kit can be used with a variety of sample types, including plasma, serum, saliva, urine, cell lysate, tissue homogenate and tissue culture medium.
Nitric oxide (NO) is a biological signaling and effector molecule involved in many biological functions, including maintaining blood pressure, modulating neural transmissions and stimulating immune responses. NO is also implicated in the regulation of hypoxia-inducible factor 1α, a transcription factor that is a key regulator of oxygen homeostasis (see TransAM™ HIF-1) (1,2). Consequently, NO has become the subject of extensive research.
Figure 1: Dynamic range of NO standard curves.
Nitrate standard curves prepared using the Nitric Oxide Quantitation Kit and a conventional LDH-method kit. The Active Motif method provides linear results over a wider range.
References
- Huang, LE et al. (1999) J. Biol. Chem. 274: 9038-9044.
- Liu, Y et al. (1998) J. Biol. Chem. 273: 15257-15262.
