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Fast Activated Cell-based ELISA (FACE™ In-Cell Western) Kits provide a simple, sensitive method for detecting protein phosphorylation directly in the cell, without making extracts or performing electrophoresis and membrane blotting. These 96-well, high-throughput assays are available in both colorimetric and chemiluminescent formats for over 20 different targets (see list at right). If you need a FACE Kit for a target that is not currently offered, you can use our FACE Maker In-Cell Western ELISA Kits to create your own FACE assays. And, the Suspension Cell FACE In-Cell Western ELISA module adapts any FACE Kit for improved results when working with suspension cells.
Why use FACE™ In-Cell Western ELISA Kits?
- Simple, non-radioactive method that is easily quantified
- Fixing preserves activation-specific protein modifications
- Cell-based assay eliminates the need to make cell extracts
- Minimal hands-on time - less than 3 hours
- Two primary antibodies to compare phosphorylated and native protein
- Increased linear analysis range
- Compatible with high-throughput automation
Figure 1: Flow chart of the FACE In-Cell Western ELISA process.
Flow chart of the FACE In-Cell Western method that uses a cell based ELISA to measure the levels of the native and phospho forms of signaling proteins and kinases that are activated by phosphorylation.
