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Fast Activated Cell-based ELISA (FACE™) Kits provide a simple, sensitive method for detecting protein phosphorylation directly in the cell, without making extracts or performing electrophoresis and membrane blotting. These 96-well, high-throughput assays are available in both colorimetric and chemiluminescent formats for over 20 different targets (see list at right). If you need a FACE Kit for a target that is not currently offered, you can use our FACE Maker Kits to create your own FACE assays. And, the Suspension Cell FACE module adapts any FACE Kit for improved results when working with suspension cells.
Why use FACE™ Kits?
- Simple, non-radioactive method that is easily quantified
- Fixing preserves activation-specific protein modifications
- Cell-based assay eliminates the need to make cell extracts
- Minimal hands-on time - less than 3 hours
- Two primary antibodies to compare phosphorylated and native protein
- Increased linear analysis range
- Compatible with high-throughput automation
