YOUR CART
Focal adhesion kinase (FAK) is a widely expressed non-receptor cytoplasmic tyrosine kinase that is implicated in integrin-mediated signal transduction. FAK plays an important role in the control of several biological processes, including cell spreading, migration and survival. Physical interactions of FAK with the integrin cytoplasmic domain and cytoskeletal proteins talin, paxillin and/or tensin play a key role in FAK activation by facilitating its oligomerization and transphosphorylation. FAK shows a rapid increase in tyrosine phosphorylation when stimulated by diverse signaling molecules, including those that regulate embryonic development, cell proliferation, migration and apoptosis, and efficient study methods are in high demand.
Contents & Storage
96-well plates for culturing cells, two primary antibodies (1 phospho-specific, 1 specific for native protein), HRP-conjugated secondary antibody, Quenching Solution, 1X Antibody Blocking Buffer, 1X Antibody Dilution Buffer, 10X PBS, 10% Triton X-100, 1% SDS Solution, Developing and Stop Solutions, and Crystal Violet Cell Quantification Solution. Storage conditions vary from room temperature to -20°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.
