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ChIP is an enrichment of DNA bound by a particular protein, not a complete purification of the protein-bound DNA. As a result, ChIPs are unavoidably contaminated with chromatin bound non-specifically. During PCR analysis, this contaminating DNA can lead to false positive PCR products; this can make data interpretation difficult and complicate the validation of antibodies and primer sets for use in ChIP. To solve these problems, Active Motif offers species-specific ChIP-IT® Control Kits that provide positive and negative control antibodies, a positive control PCR primer set as well as a PCR buffer/DNA loading dye that makes your PCR reactions ready to run on the gel straight out of the thermocycler. No matter what your sample or which ChIP-IT Kit you use, our control kits will help ensure your success.
| Name | Format | Cat No. | Price | |
|---|---|---|---|---|
| ChIP-IT® Control Kit – Human | 5 rxns | 53010 | $100 | Buy Now |
| ChIP-IT® Control Kit – Mouse | 5 rxns | 53011 | $100 | Buy Now |
| ChIP-IT® Control Kit – Rat | 5 rxns | 53012 | $100 | Buy Now |
ChIP-IT Control Kits provide positive & negative control antibodies and species-specific PCR primers, PCR buffer and a convenient DNA loading dye that makes your PCR reactions ready for loading on an agarose gel straight from the thermocycler.
Contents & Storage
Five ChIP reactions each of Positive Control antibody, Negative Control IgG, species-specific positive control PCR primers, 10X PCR Buffer and 10X PCR loading dye. Reagent storage conditions vary from 4°C to -20°C. All reagents are guaranteed stable for 6 months when stored properly.
