YOUR CART
In response to stress, the expression level of HSP27 increases several fold to confer cellular resistance to the adverse environmental change. HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAPK-2 as a result of the activation of the p38 MAPK pathway. Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers. Thus, phosphorylated HSPs have significantly decreased ability to act as molecular chaperones. Phosphorylation of HSP27 on Ser82 by MAPKAPK-2 or AKT leads to HSP dissociation from the AKT/MAPKAPK2/p38 complex, which may promote independent survival signals. The phosphorylation of HSP27 also enhances its association with IKKβ to result in decreased IKK activity.
Contents & Storage
96-well plates for culturing cells, two primary antibodies (1 phospho-specific, 1 specific for native protein), HRP-conjugated secondary antibody, Quenching Solution, 1X Antibody Blocking Buffer, 1X Antibody Dilution Buffer, 10X PBS, 10% Triton X-100, 1% SDS Solution, Developing and Stop Solutions, and Crystal Violet Cell Quantification Solution. Storage conditions vary from room temperature to -20°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.
