fast, accurate quantification of phosphorylated IκBα (S32/S36)
The FunctionELISA IκBα Kit offers a fast, simple method to monitor the phosphorylation of IκBα, an event required for NFκB translocation. The kit utilizes the Sandwich ELISA technique to capture and quantifiably measure the amount of phospho-IκBα present in a sample, making the kit useful in monitoring the activation of NFκB and the many genes it regulates. Click the IκBα Info tab below for data and more information.
FunctionELISA IκBα comes ready-to-use, with Capture Antibody specific for the phosphorylated form of IκBα already bound to the plate; this saves you the time and inconvenience of performing overnight incubations. The kit employs highly sensitive chemiluminescent detection, so requires the use of a luminometer. For more information, click the Method & Contents tabs below.
|FunctionELISA™ IκBα||1 x 96 rxns||48005||$565||Buy Now|
|5 x 96 rxns||48505||$2,275||Buy Now|
|FunctionELISA™ IκBα Manual|
|Gene Regulation Products & Services Brochure|
|MSDS: Sodium Azide|
|MSDS: Sulphuric Acid|
FunctionELISA IκBα includes a Capture Antibody specific for IκBα that has been phosphorylated at serines 32,36. It utilizes highly sensitive chemiluminescent detection that enables accurate study of the phosphorylation state of IκBα in response to external stimuli (Figure 1). Because of the chemiluminescent detection method, the FunctionELISA IκBα Kit requires the use of a luminometer.
Figure 1: Induction of IκBα phosphorylation.
NFκB is regulated by the IκB family of inhibitory proteins. Phosphorylation of IκBα can be triggered by a wide range of extracellular signals, including cytokines and chemokines. It leads, ultimately, to activation of NFκB. Thus, analysis of the phosphorylation state of IκBα provides insights about NFκB and the many genes it regulates. (For additional tools for the study of NFκB, see TransAM® NFκB p50, p52, p65 and Family Kits and NFκB-related antibodies).
Why use FunctionELISA?
- Quantitative results in just hours
- ELISA format eliminates need to run, blot and develop gels
- Analyze multiple samples in low-volume, high-throughput experiments
- Complete kit with all required reagents and controls
- Ready-to-use format with capture antibody pre-coated on the plate
- Ability to assay both cell and tissue samples
Sandwich ELISA method
FunctionELISA IκBα utilizes the Sandwich ELISA technique to capture and quantifiably measure the amount of phosphorylated IκBα present in a sample. Sandwich ELISAs use two antibodies that recognize different epitopes on the protein being studied. The first antibody is immobilized in the wells of an ELISA plate. When cell lysate is added, the specific protein of interest is bound by this Capture Antibody. A second antibody, called the Detecting Antibody, is then added. It binds to the bound protein. A horseradish peroxidase (HRP)-conjugated secondary antibody binds to the Detecting Antibody to quantify the amount of protein bound by the Capture Antibody (Figure 1).
Figure 1: Sandwich ELISA schematic.
Contents & Storage
One or five 96-well assay plates coated with Capture Antibody, Detecting Antibody, HRP-conjugated Secondary Antibody, Lysis and Wash Buffers, Protease Inhibitor, DTT, and Control protein. The FunctionELISA IκBα Kit requires the use of a luminometer.