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NFκB is regulated by the IκB family of inhibitory proteins. Phosphorylation of IκBα can be triggered by a wide range of extracellular signals, including cytokines and chemokines. It leads, ultimately, to activation of NFκB. Thus, analysis of the phosphorylation state of IκBα provides insights about NFκB and the many genes it regulates. (For additional tools for the study of NFκB, see TransAM™ NFκB p50, p52, p65 and Family Kits and NFκB-related antibodies).
Contents & Storage
One or five 96-well assay plates coated with Capture Antibody, Detecting Antibody, HRP-conjugated Secondary Antibody, Lysis and Wash Buffers, Protease Inhibitor, DTT, and Control protein. The FunctionELISA IκBα Kit requires the use of a luminometer.
