Your Cart
Browse
Filter
FunctionELISA™ Kits offer a rapid, simple method to monitor changes in signal pathway proteins. They use the Sandwich ELISA technique to capture and quantifiably measure the amount of a specific protein present in a sample. FunctionELISA Kits come ready-to-use, with the Capture Antibody already bound to the plate, saving you the time and inconvenience of performing overnight incubations. For complete details, click the Method tab below.
FunctionELISA IκBα provides Capture Antibody that is specific for the phosphorylated form of IκBα, making the kit useful in monitoring the activation of NFκB and the many genes it regulates. It utilizes highly sensitive chemiluminescent detection, so requires the use of a luminometer. Click the IκBα Info tab below for data and more information.
| Name | Format | Cat No. | Price | |
|---|---|---|---|---|
| FunctionELISA™ IκBα | 1 x 96 rxns | 48005 | $550 | Buy Now |
| 5 x 96 rxns | 48505 | $2,225 | Buy Now |
| FunctionELISA™ IκBα Manual |
| FunctionELISA™ Profile |
| Cell Biology Products Brochure |
| MSDS: Sodium Azide |
| MSDS: Sulphuric Acid |
| MSDS: Thimersol |
FunctionELISA IκBα uses Capture Antibody specific for phosphorylated IκBα at serines 32,36 and highly sensitive chemiluminescent detection to enable accurate study of the phosphorylation state of IκBα in response to external stimuli (Figure 1). This FunctionELISA Kit requires the use of a luminometer.
Figure 1: Induction of IκBα phosphorylation.
Jurkat cells were stimulated with 1 nM TNF-α and harvested at the indicated time points. Whole-cell lysate was assayed using Western blot analysis with Active Motif’s IκBα phospho Ser32,36 mAb (Catalog No. 40904) (top gel) and the FunctionELISA IκBα Kit (bottom graph). The FunctionELISA Kit clearly provides more quantitative results than are possible with Western.
IκBα Overview
NFκB is regulated by the IκB family of inhibitory proteins. Phosphorylation of IκBα can be triggered by a wide range of extracellular signals, including cytokines and chemokines. It leads, ultimately, to activation of NFκB. Thus, analysis of the phosphorylation state of IκBα provides insights about NFκB and the many genes it regulates. (For additional tools for the study of NFκB, see TransAM® NFκB p50, p52, p65 and Family Kits and NFκB-related antibodies).
FunctionELISA Kits utilize the Sandwich ELISA technique to capture and quantifiably measure the amount of a specific protein present in a sample. Sandwich ELISAs use two antibodies that recognize different epitopes on the protein being studied. The first antibody is immobilized in the wells of an ELISA plate. When cell lysate is added, the specific protein of interest is bound by this Capture Antibody. A second antibody, called the Detecting Antibody, is then added. It binds to the bound protein. The FunctionELISA IκBα and TRAIL Kits use a horseradish peroxidase (HRP) or alkaline phosphatase (AP) conjugated secondary antibody, respectively, that binds to the Detecting Antibody to quantify the amount of protein bound by the Capture Antibody (Figure 1).
Figure 1: Sandwich ELISA schematics.
In FunctionELISA Kits, Capture and Detecting Antibodies are used for sensitive, accurate quantification of the antigen of interest (Ag). FunctionELISA IκBα and TRAIL Kits use a horseradish peroxidase (HRP) or alkaline phosphatase (AP) conjugated secondary antibody, respectively, that binds to the Detecting Antibody to quantify the amount of protein bound by the Capture Antibody.
Why use FunctionELISA?
- Quantitative results in just hours
- ELISA format eliminates need to run, blot and develop gels
- Analyze multiple samples in low-volume, high-throughput experiments
- Complete kit with all required reagents and controls
- Ready-to-use format with capture antibody precoated on the plate
- Ability to assay both cell and tissue samples
Contents & Storage
One or five 96-well assay plates coated with Capture Antibody, Detecting Antibody, HRP-conjugated Secondary Antibody, Lysis and Wash Buffers, Protease Inhibitor, DTT, and Control protein. The FunctionELISA IκBα Kit requires the use of a luminometer.
