sandwich ELISA for accurate quantification of TRAIL
FunctionELISA™ Kits offer a rapid, simple method to monitor changes in signal pathway proteins. They use the Sandwich ELISA technique to capture and quantifiably measure the amount of a specific protein present in a sample. FunctionELISA Kits come ready-to-use, with the Capture Antibody already bound to the plate, saving you the time and inconvenience of performing overnight incubations. For complete details, click the Method tab below.
FunctionELISA TRAIL provides Capture Antibody that is specific for Tumor Necrosis Factor Related Apoptosis Inducing Ligand, a TNF Family member that appears to induce apoptosis in cancer cells, but is not toxic to normal cells. Click the TRAIL Info tab below for data and more information.
|FunctionELISA™ TRAIL||1 x 96 rxns||48010||$550||Buy Now|
|5 x 96 rxns||48510||$2,225||Buy Now|
|FunctionELISA™ TRAIL Manual|
|Cell Biology Products Brochure|
|MSDS: Sodium Azide|
|MSDS: Sulphuric Acid|
FunctionELISA TRAIL can accurately measure picogram quantities of the soluble form of TRAIL in lysates and fluid samples. This FunctionELISA Kit utilizes an AP-conjugated secondary antibody and requires the use of a spectrophotometer.
Figure 1: Time course of TRAIL induction.
Tumor Necrosis Factor-Related Apoptosis Inducing Ligand (TRAIL) is a member of the TNF ligand family. It can induce apoptosis in a wide range of cancer cells with no toxicity on most normal cells. TRAIL may also play roles in immune responses, autoimmune disorders and AIDS.
FunctionELISA Kits utilize the Sandwich ELISA technique to capture and quantifiably measure the amount of a specific protein present in a sample. Sandwich ELISAs use two antibodies that recognize different epitopes on the protein being studied. The first antibody is immobilized in the wells of an ELISA plate. When cell lysate is added, the specific protein of interest is bound by this Capture Antibody. A second antibody, called the Detecting Antibody, is then added. It binds to the bound protein. The FunctionELISA IκBα and TRAIL Kits use a horseradish peroxidase (HRP) or alkaline phosphatase (AP) conjugated secondary antibody, respectively, that binds to the Detecting Antibody to quantify the amount of protein bound by the Capture Antibody (Figure 1).
Figure 1: Sandwich ELISA schematics.
Why use FunctionELISA?
- Quantitative results in just hours
- ELISA format eliminates need to run, blot and develop gels
- Analyze multiple samples in low-volume, high-throughput experiments
- Complete kit with all required reagents and controls
- Ready-to-use format with capture antibody precoated on the plate
- Ability to assay both cell and tissue samples
Contents & Storage
One or five 96-well assay plates coated with Capture Antibody, Detecting Antibody, AP-Conjugated Secondary Antibody, Lysis and Wash Buffers, BSA, Recombinant protein and pNPP Substrate Solutions.