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Protein Detection and Quantitation

FunctionELISA™ TRAIL

sandwich ELISA for accurate quantification of TRAIL

FunctionELISA™ Kits offer a rapid, simple method to monitor changes in signal pathway proteins. They use the Sandwich ELISA technique to capture and quantifiably measure the amount of a specific protein present in a sample. FunctionELISA Kits come ready-to-use, with the Capture Antibody already bound to the plate, saving you the time and inconvenience of performing overnight incubations. For complete details, click the Method tab below.

FunctionELISA TRAIL provides Capture Antibody that is specific for Tumor Necrosis Factor Related Apoptosis Inducing Ligand, a TNF Family member that appears to induce apoptosis in cancer cells, but is not toxic to normal cells. Click the TRAIL Info tab below for data and more information.

 
Name Format Cat No. Price  
FunctionELISA™ TRAIL 1 x 96 rxns 48010 $550 Buy Now
5 x 96 rxns 48510 $2,225 Buy Now

FunctionELISA TRAIL can accurately measure picogram quantities of the soluble form of TRAIL in lysates and fluid samples. This FunctionELISA Kit utilizes an AP-conjugated secondary antibody and requires the use of a spectrophotometer.

 
FunctionELISA TRAIL and Western blot methods used to monitor the time course of TRAIL accumulation in TNF-induced Jurkat cells
 
Figure 1: Time course of TRAIL induction.

Jurkat cells were stimulated with 1 nM TNF-α and harvested at the indicated time points. Whole-cell lysate was assayed by Western blot using TRAIL mAb (Catalog No. 40966) (top gel) and in a FunctionELISA TRAIL assay (bottom graph). The FunctionELISA Kit clearly provides more quantitative data than is possible with Western.

TRAIL Info

Tumor Necrosis Factor-Related Apoptosis Inducing Ligand (TRAIL) is a member of the TNF ligand family. It can induce apoptosis in a wide range of cancer cells with no toxicity on most normal cells. TRAIL may also play roles in immune responses, autoimmune disorders and AIDS.

FunctionELISA Kits utilize the Sandwich ELISA technique to capture and quantifiably measure the amount of a specific protein present in a sample. Sandwich ELISAs use two antibodies that recognize different epitopes on the protein being studied. The first antibody is immobilized in the wells of an ELISA plate. When cell lysate is added, the specific protein of interest is bound by this Capture Antibody. A second antibody, called the Detecting Antibody, is then added. It binds to the bound protein. The FunctionELISA IκBα and TRAIL Kits use a horseradish peroxidase (HRP) or alkaline phosphatase (AP) conjugated secondary antibody, respectively, that binds to the Detecting Antibody to quantify the amount of protein bound by the Capture Antibody (Figure 1).

 
Flow chart of the FunctionELISA method for quantifying signaling proteins.
 
Figure 1: Sandwich ELISA schematics.

In FunctionELISA Kits, Capture and Detecting Antibodies are used for sensitive, accurate quantification of the antigen of interest (Ag). FunctionELISA IκBα and TRAIL Kits use a horseradish peroxidase (HRP) or alkaline phosphatase (AP) conjugated secondary antibody, respectively, that binds to the Detecting Antibody to quantify the amount of protein bound by the Capture Antibody.

Why use FunctionELISA?

  • Quantitative results in just hours
  • ELISA format eliminates need to run, blot and develop gels
  • Analyze multiple samples in low-volume, high-throughput experiments
  • Complete kit with all required reagents and controls
  • Ready-to-use format with capture antibody precoated on the plate
  • Ability to assay both cell and tissue samples

Contents & Storage

One or five 96-well assay plates coated with Capture Antibody, Detecting Antibody, AP-Conjugated Secondary Antibody, Lysis and Wash Buffers, BSA, Recombinant protein and pNPP Substrate Solutions.