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Active Motif's SUMOlink™ Kits provide a simple method for generating SUMOylated proteins in vitro. Using the SUMOylation enzyme cascade, carboxy-terminal SUMO-1, SUMO-2 or SUMO-3 peptide substrates are linked covalently to specific lysine residues on the target protein via isopeptide bonds. SUMOlink Kits provide all the reagents required for performing in vitro SUMOylation of target proteins, including positive and negative controls that help ensure success.
Contents & Storage
SUMO-1 or SUMO-2 and SUMO-3 wild-type and mutated proteins, SUMO-1 or SUMO-2/3 antibodies, p53 control protein, p53 antibody, SUMOylation buffer, Protein buffer, E1 activating enzyme and E2 conjugating enzyme. Reagent storage conditions vary from -20°C to -80°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.
SUMOlink Kits provide enough reagents to perform 20 in vitro SUMOylations with the WT SUMO protein and 20 in vitro SUMOylations with the Mutated SUMO protein.
