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ChIP-IT™ Kits make performing chromatin immunoprecipitation (ChIP) more successful by combining all of the critical components needed for ChIP in a single kit. For your convenience, kits are available with your choice of sonication or our exclusive enzymatic shearing, and either with or without human positive & negative controls.
Flexible ordering options
The ChIP-IT and ChIP-IT Enzymatic Kits (both with and without controls) include Salmon Sperm DNA/Protein G agarose and the shearing components found in the ChIP-IT Shearing or Enzymatic Shearing Kits, respectively. However, depending on how many immunoprecipitations you perform with each sample of sheared chromatin, you may run out of shearing components before using up all of the immunoprecipitation components in the ChIP-IT Kits. For this reason, the ChIP-IT Sonication & Enzymatic Shearing Kits and the Protein G Magnetic & Agarose Beads are also sold separately.
ChIP-IT Kits provide sufficient components to perform 25 ChIP reactions, and sufficient shearing components to optimize shearing conditions and then make 5 preparations of sheared chromatin from three 15 cm plates (4.5 x 107 cells); each chromatin preparation will yield enough material to perform approximately 16 ChIP reactions. Kits that contain controls include sufficient positive & negative control antibodies and human-specific PCR primer sets to perform 5 control ChIP reactions each.
Contents & Storage
Pre-blocked Protein G agarose, Shearing Buffer (or Digestion Buffer and Enzymatic Shearing Cocktail), ChIP Antibody Binding, DNA Binding, Wash and 10X PCR Buffers, 10X PBS, 10X Glycine, 1X Lysis Buffer, Protease Inhibitor Cocktail, PMSF, EDTA, Proteinase K, RNase A, DNA purification columns and buffers, and Positive Control antibody, Negative Control IgG, and human-specific positive & negative control PCR primer sets. Reagent storage conditions vary from room temperature to -20°C. Please download a manual for complete kit specifications and storage conditions. All reagents are guaranteed stable for 6 months when stored properly.
