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ChIP-IT™ Kits make performing chromatin immunoprecipitation (ChIP) more successful by combining all of the critical components needed for ChIP in a single kit. For your convenience, kits are available with your choice of sonication or our exclusive enzymatic shearing, and either with or without human positive & negative controls.
ChIP-IT Kits provide all of the critical components needed for successful ChIP in a single kit. ChIP-IT comes complete with positive & negative control antibodies and PCR primers, DNA purification columns, optimized buffers and a comprehensive manual, all of which have been proven to work in ChIP. For your convenience, kits are available in a variety of formats, with your choice of sonication or our exclusive enzymatic shearing, and either with or without positive & negative controls. This enables you to use the shearing method that works best for you, and to either receive human-specific controls with the kit or to purchase ChIP-IT Control Kits separately to help analyze your results and verify that your antibodies work in the species you choose.
Choose from sonication or reproducible enzymatic shearing
The first step in successful ChIP is shearing the chromatin into 200-1000 bp fragments. This has traditionally been performed using sonication. Although sonication is effective, many users find that complications caused by emulsification and overheating can lead to variable results. To improve reproducibility, Active Motif has developed a unique enzymatic shearing method. For your convenience, all ChIP-IT and ChIP-IT Enzymatic Kits are available in your choice of sonication or enzymatic shearing formats and stand-alone kits are also available. Click here to learn more about your options. And, to make you ChIP even simpler, we also offer Ready-to-ChIP Chromatin. Using our chromatin and ChIP-IT reagents, the only variable in your ChIP will be the antibody you are testing.
Get only the controls you need
ChIP-IT positive and negative controls simplify the task of troubleshooting your experiment, interpreting your results, and validating antibodies for use in ChIP. However, once an antibody has been shown to work in ChIP, it may no longer be necessary to run the controls that are included in the original ChIP-IT Kits. To prevent wasted reagents and unnecessary cost, Active Motif also offers its ChIP-IT Kits without the control reagents. (Likewise, if you are working with a non-human samples, instead of receiving the human controls included in the ChIP-IT Kits, you may want to purchase the ChIP-IT Kit w/o controls together with a ChIP-IT Control Kit for mouse or rat samples.) So, if you already have ChIP-validated antibodies, or if you're working with non-human samples, you can get exactly what you want, without having to pay for controls that you may not need. In addition, Active Motif offers an ever-increasing number of ChIP-validated antibodies, which are ideal for use with ChIP-IT.
