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Now you can easily purify histones and further separate the fractions of core histones from any cell culture or tissue sample while maintaining post-translational modifications like acetylation, methylation and phosphorylation states. The Histone Purification Kit enables you to isolate core histones from any cell culture or tissue sample. The core histones may be purified using the convenient spin column format as one total population containing H2A, H2B, H3 and H4, or further purified into separate fractions of H2A/H2B dimers and H3/H4 tetramers with the efficient gravity flow protocol.
Contents & Storage
10 purification reactions each of 5X Neutralization Buffer, Extraction Buffer, Equilibration Buffer, Histone Wash Buffer, H2A/H2B Elution Buffer, H3/H4 Elution Buffer, Purification Resin and Purification Column. Reagents are to be stored at 4°C. All reagents are guaranteed stable for 6 months from date of receipt when stored properly.
