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AML/Runx factors are essential for blood, skeletal and gastric development and are composed of heterodimeric α and β subunits. In mammals, the α subunits are encoded by three genes, Runx1, 2 and 3, while the β subunit (PEBP2β, CBFβ) is ubiquitously expressed. AML-1/Runx1 is essential for definitive hematopoiesis and is one of the most frequently mutated genes in human leukemia development, while AML-3/Runx2 controls osteoblast differentiation and the development of hypertrophic cartilage. Mutations of AML-3 lead to the rare bone disease cleidocranial dysplasia (CCD) and are associated with human leukemia.
Figure 1: Monitoring AML-3/Runx2 activation with the TransAM AML-3/Runx2 Kit.
Different amounts of nuclear extracts from unstimulated HeLa and HL-60 cells are tested for AML-3/Runx2 activation by using the TransAM AML-3/Runx2 Kit.
