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AP-1 proteins play a role in the expression of many genes involved in cellular proliferation and cell cycle progression. The transcription factor AP-1 is composed of a mixture of heterodimeric protein complexes derived from the Fos and Jun families, including c-Fos, FosB, Fra-1, Fra-2, c-Jun, JunB and JunD. AP-1 heterodimers bind to DNA on a serum response element with the 5´-TGA(C/G)TCA-3´ sequence. Jun proteins can bind to form transcriptionally active homodimers or heterodimers with CREB/ATF members to bind the CRE element. AP-1 expression is induced by multiple stimuli, including the presence of serum, growth factors, phorbol esters and oncogenes. Phosphorylation of AP-1 family members by kinases is required for transactivation activity.
Contents & Storage
One, two or five AP-1 96-well plate(s) with plate sealer(s), c-Fos, FosB, c-Jun, JunD or c-Fos, FosB, Fra-1, Fra-2, c-Jun, JunB and JunD primary antibody(ies), HRP-conjugated secondary antibody, AP-1 wild-type and mutated oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Poly[d(I-C)], Lysis, Binding, 10X Washing and 10X Antibody Binding Buffer, and Developing and Stop Solutions. Reagent storage conditions vary from room temperature to -80°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.
