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AP-1 proteins play a role in the expression of many genes involved in cellular proliferation and cell cycle progression. The transcription factor AP-1 is composed of a mixture of heterodimeric protein complexes derived from the Fos and Jun families, including c-Fos, FosB, Fra-1, Fra-2, c-Jun, JunB and JunD. AP-1 heterodimers bind to DNA on a serum response element with the 5´-TGA(C/G)TCA-3´ sequence. Jun proteins can bind to form transcriptionally active homodimers or heterodimers with CREB/ATF members to bind the CRE element. AP-1 expression is induced by multiple stimuli, including the presence of serum, growth factors, phorbol esters and oncogenes. Phosphorylation of AP-1 family members by kinases is required for transactivation activity.
TransAM™ AP-1 Kits are available with antibodies specific for the activated form of c-Fos, FosB, JunD or phosphorylated c-Jun. A family kit is also available with the ability to independently screen for all the members of the AP-1 family simultaneously (Figure 1).
Variety of kits available
- c-Fos
- FosB
- c-Jun
- JunD
- Family Kit (c-Fos, FosB, Fra-1, Fra-2, c-Jun, JunB and JunD)
Figure 1: AP-1 family profiling for DNA binding activation upon serum addition.
Nuclear extracts from HeLa cells (untreated or stimulated by serum addition following serum starvation, SR) are assayed at 5 µg/well for c-Fos, FosB, Fra-1, Fra-2, c-Jun, JunB and JunD activity using the TransAM AP-1 Family Kit.
