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ATF-2 is a member of the ATF/CREB family that binds to the cAMP response element (CRE). ATF-2 is activated by ERK1/2, JNK and p38. TransAM ATF-2 Kits contain an antibody specific for the phosphorylated form of ATF-2 when bound to its target DNA. Because of ATF's regulation by MAPK, we also offer the TransAM MAPK Family Kit for studying the MAPK regulated transcription factors ATF-2, c-Jun, c-Myc, MEF2 and STAT1α.
Contents & Storage
One or five ATF 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions. Reagent storage conditions vary from room temperature to -80°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.
