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The family of C/EBP (CCAAT-enhancer binding protein) basic leucine zipper transcription factors includes C/EBPα, C/EBPβ, C/EBPδ, C/EBPγ and C/EBPε. Each member exhibits similar DNA-binding specificities and contains a leucine zipper dimerization region. C/EBPs play an important role in regulating the balance between cell growth and differentiation. C/EBP expression is, therefore, a function of tissue type as well as temporal period of development. The TransAM C/EBP α/β Kit contains antibodies specific for the active form of C/EBPα and C/EBPβ when bound to the target DNA.
Contents & Storage
One or five C/EBP α/β 96-well plate(s) with plate sealer(s), C/EBPα and C/EBPβ primary antibodies, HRP-conjugated secondary antibody, C/EBP wild-type and mutated oligonucleotides, positive control cell extract, DTT, Herring Sperm DNA, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions. Reagent storage conditions vary from room temperature to -80°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.
