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Glucocorticoids play an essential role in maintaining basal and stress-related homeostasis. Glucocorticoid Receptor (GR) is the mediator for most known effects of glucocorticoids. Abnormalities in GR sensitivity can lead to steroid-resistant asthma, rheumatoid arthritis, osteoarthritis, hypertension and obesity. Because of the importance of GR in maintaining homeostasis, accurate monitoring of GR in cells, tissues and animals is crucial for many biomedical research and drug development projects.
Figure 1: Monitoring GR activity with the TransAM GR Kit.
Different amounts of nuclear extracts from untreated and Dexamethasone-treated HeLa cells are tested for GR activity using the TransAM GR Kit.
