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Hepatocyte Nuclear Factors (HNFs) are primarily required for cellular differentiation and metabolism. Unlike most transcription factor families, this group of evolutionarily conserved proteins does not have a common consensus-binding site. This makes assaying HNF family activity using classical EMSA or reporter assays cumbersome and time consuming. The TransAM HNF Family Kit contains multiple binding sites in each well, making it quick and easy to monitor HNF-1, -3α, -3β and -4α activity in a single assay. In addition, a TransAM HNF-1 Kit is available separately for researchers who want to monitor this important regulator of liver function.
Contents & Storage
One or five HNF-1 96-well plate(s) or two HNF Family 96-well plates with plate sealer(s), HNF-1 or HNF-1, HNF-3α, HNF-3β and HNF-4α primary antibodies, HRP-conjugated secondary antibody, HNF-1 or HNF family wild-type and mutated oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions. Reagent storage conditions vary from room temperature to -80°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.
