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Hepatocyte Nuclear Factors (HNFs) are primarily required for cellular differentiation and metabolism. Unlike most transcription factor families, this group of evolutionarily conserved proteins does not have a common consensus-binding site. This makes assaying HNF family activity using classical EMSA or reporter assays cumbersome and time consuming. The TransAM HNF Family Kit contains multiple binding sites in each well, making it quick and easy to monitor HNF-1, -3α, -3β and -4α activity in a single assay. In addition, a TransAM HNF-1 Kit is available separately for researchers who want to monitor this important regulator of liver function.
Applications
TransAM HNF-1 and HNF Family Kits can be used in investigations of HNF involvement in metabolism, liver development and glucose homeostasis. Applications include:
- Drug screening and/or potency studies
- Monitoring of HNF transcriptional activity regulation
- Protein structure/function studies of HNF
Figure 1: Monitoring HNF-1 activity with the TransAM HNF-1 Kit.
Different amounts of nuclear extracts from untreated Hep G2 and HeLa cells are tested for HNF-1 activity using the TransAM HNF-1 Kit.
