YOUR CART
The activation of mitogen-activated protein kinase (MAPK) signal transduction pathways is responsible for the phosphorylation of transcription factors by the terminal kinase in these cascades. MAPK pathways play major roles in converting mitogenic and stress stimuli into nuclear responses, Therefore, accurate monitoring of MAPK substrates in cells, tissues and animals is crucial for many biomedical research and drug development projects. The TransAM MAPK Family Kit contains multiple binding sites in each well and includes antibodies specific for the active forms of the transcription factors: phosphorylated ATF-2, phosphorylated c-Jun, c-Myc, MEF2 and STAT1α, all of which are regulated by MAP kinase cascades and are key markers in cell signaling. In addition to the TransAM MAPK Family Kit, individual kits for studying ATF-2, c-Jun, c-Myc and MEF2 are also available.
Contents & Storage
Two MAPK Family 96-well plates with plate sealer(s), phosphorylated ATF-2, phosphorylated c-Jun, c-Myc, MEF2 and STAT1α primary antibody(ies), HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions. Reagent storage conditions vary from room temperature to -80°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.
