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The activation of mitogen-activated protein kinase (MAPK) signal transduction pathways is responsible for the phosphorylation of transcription factors by the terminal kinase in these cascades. MAPK pathways play major roles in converting mitogenic and stress stimuli into nuclear responses, Therefore, accurate monitoring of MAPK substrates in cells, tissues and animals is crucial for many biomedical research and drug development projects. The TransAM MAPK Family Kit contains multiple binding sites in each well and includes antibodies specific for the active forms of the transcription factors: phosphorylated ATF-2, phosphorylated c-Jun, c-Myc, MEF2 and STAT1α, all of which are regulated by MAP kinase cascades and are key markers in cell signaling. In addition to the TransAM MAPK Family Kit, individual kits for studying ATF-2, c-Jun, c-Myc and MEF2 are also available.
Applications
MAPK regulated genes are involved in a variety of cellular pathways that are currently being studied for new drug targets. Applications include:
- Drug screening and/or potency studies
- Monitoring of transcriptional activity regulation
- Protein structure/function studies of MAPK regulated genes
Figure 1: TransAM profiling of MAPK-regulated transcription factors.
The TransAM MAPK Family Kit was used to assay the activation levels of ATF-2, c-Myc, c-Jun, STAT1α and MEF2 using nuclear extracts prepared from either HeLa (TPA stimulated), Jurkat (unstimulated), K-562 (TPA stimulated) or U-937 (TPA + IFNγ treated) cells.
