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ChIP-IT™ Kits make performing chromatin immunoprecipitation (ChIP) more successful by combining all of the critical components needed in a single kit, including shearing components. However, depending on how many immunoprecipitations you perform with each sample of sheared chromatin, you may run out of shearing components before using up all of the immunoprecipitation components in the ChIP-IT Kits. For this reason, the ChIP-IT Sonication & Enzymatic Shearing Kits are also sold separately. This enables you to buy only the reagents you need, while giving you the flexibility to use either traditional sonication or our unique enzymatic shearing.
Flexible ordering options
The ChIP-IT Sonication and Enzymatic Shearing Kits provide sufficient shearing components to optimize shearing conditions and then make 5 preparations of sheared chromatin from three 15 cm plates (4.5 x 107 cells); each chromatin preparation will yield enough material to perform approximately 16 ChIP reactions. (These identical shearing components are already included in the ChIP-IT and ChIP-IT Express Kits, so you don’t need to buy a stand-alone shearing kit unless you’re running out of shearing components.)
Contents & Storage
Shearing Buffer (or Digestion Buffer and Enzymatic Shearing Cocktail), 10X PBS, 10X Glycine, 1X Lysis Buffer, Protease Inhibitor Cocktail, PMSF, EDTA, Proteinase K and RNase A. Reagent storage conditions vary from room temperature to -20°C. Please download a manual for complete kit specifications and storage conditions. All reagents are guaranteed stable for 6 months when stored properly.
