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NF-Y is a ubiquitously expressed heterodimeric transcription factor composed of three subunits (NF-YA, NF-YB and NF-YC). All three subunits are required for constitution of a functional binding complex to the CCAAT box. CCAAT boxes are found in the regulatory region of 30% of genes, including housekeeping genes as well as inducible and cell cycle-regulated genes. The role of a CCAAT box as a cis-acting promoter element depends on its ability to interact with NF-Y. Methods to measure overall levels and modifications of the NF-YA subunit are crucial to understanding transcriptional regulation of the numerous CCAAT box-containing genes. The TransAM NF-YA Kit contains an antibody specific for the active form of the NF-YA subunit when it is bound to the target DNA.
Contents & Storage
One or five NF-YA 96-well assay plate(s) with plate sealer(s), NF-YA primary antibody, HRP-conjugated secondary antibody, NF-Y wild-type and mutated oligonucleotides, positive control cell extract, Dithiothreitol, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, Developing and Stop Solutions and Herring Sperm DNA. Reagent storage conditions vary from room temperature to -80°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.
