YOUR CART
The transcription factor NFκB (nuclear factor κB) is implicated in the regulation of many genes that code for mediators of the immune, acute phase and inflammatory responses. NFκB exists in an inactive form bound to the inhibitory IκB proteins in the cytoplasm. Treatment of cells with various inducers, such as lipopolysaccharide, TNF or IL-1, results in the degradation of the IκB proteins (See the FunctionELISA™ IκBα Kit). This releases NFκB dimers, which subsequently translocate to the nucleus where they activate appropriate target genes. Homo- and heterodimers of members of the Rel/NFκB family specifically recognize the 5´-GGGACTTTCC-3´ nucleotide sequence. The p50/p65 heterodimers and the p50 homodimers are the most common dimers found in the NFκB signaling pathway.
Contents & Storage
One, two or five NFκB 96-well assay plate(s) with plate sealer(s), NFκB p50, p52 or p65 or p50, p52, p65, c-Rel and RelB primary antibody(ies), HRP-conjugated secondary antibody, NFκB wild-type and mutated oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Herring Sperm DNA, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing & Stop Solutions. Reagent storage conditions vary from room temperature to -80°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.
* Technology covered under EAT-filed patents and licensed to Active Motif. Use of TransAM in NFκB-related drug discovery may be covered under U.S. Patent No. 6,150,090 and require a license from Ariad Pharmaceuticals (Cambridge, MA, USA).
