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The TransAM Flexi format enables you to look at NFκB binding at sites of your own design. This Kit works just like the other TransAM Kits, but you generate your own biotinylated oligo to capture activated NFκB binding. If you are interested in detecting NFκB family members binding to the traditional NFκB consensus sequence, please take a look at our other TransAM NFκB Kits.
Contents & Storage
TransAM Flexi Kits are designed for customers interested in studying alternative binding sites of NFκB and require you to design and synthesize your own oligos of interest, which will then be coated onto the plate. Enough control biotinylated oligo, which contains the consensus-binding site used in the original TransAM NFκB Kits, is provided to perform 40 control reactions. If you are interested in looking at only the consensus site of NFκB, please use the original TransAM NFκB Kits, as they conveniently provide the oligo precoated to the plate. Flexi Kits also include one or two 96-well streptavidin-coated assay plate(s) with plate sealer(s), NFκB p50 or p65 or p50, p52, p65, c-Rel and RelB primary antibody(ies), HRP-conjugated secondary antibody, NFκB wild-type and mutated oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Herring Sperm DNA, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing Solutions. Reagent storage conditions vary from room temperature to -80°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.
* Technology covered under EAT-filed patents and licensed to Active Motif. Use of TransAM in NFκB-related drug discovery may be covered under U.S. Patent No. 6,150,090 and require a license from Ariad Pharmaceuticals (Cambridge, MA, USA).
