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The TransAM Flexi format enables you to look at NFκB binding at sites of your own design. This Kit works just like the other TransAM Kits, but you generate your own biotinylated oligo to capture activated NFκB binding. If you are interested in detecting NFκB family members binding to the traditional NFκB consensus sequence, please take a look at our other TransAM NFκB Kits.
The TransAM Flexi format enables you to study NFκB binding at any binding site (Figure 1) to investigate alternative binding sites for NFκB. With this Kit, you simply provide your own biotinylated oligo to capture activated NFκB binding.
Applications
TransAM Flexi Kits are ideal for studying alternative binding sites for NFκB. Applications include:
- Study of variant transcription factor binding sites
- Analysis of native promoters
- Confirmation of chromatin immunoprecipitation (ChIP) results
- Determination of isoform-binding affinity for NFκB
Variety of kits and formats
- Colorimetric (p50 and p65)
- Chemiluminescent (p50 and p65)
- Family Kit (p50, p52, p65 (RelA), c-Rel and RelB)
- Flexi (p50, p65 and family)
Features
- Results in under 5 hours
- 10-fold more sensitive than Gelshift assays
- Quantitative results from cell or tissue extracts
- High-throughput compatible
- Non-radioactive assay
- No cell transfections required
Figure 1: Measuring NFκB p65 at different binding sites.
Five µg of nuclear extract from untreated and TNF-α-treated HeLa cells were used to assay the binding affinity of NFκB p65 for four different biotinylated 50-mer oligos. Each of the 3 test oligos (Igκ, IL-8 and uPA) contained the wild-type binding site of a promoter regulated by NFκB. These sites were compared to the consensus-binding site control provided in TransAM Flexi NFκB Kits.
