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The TransAM Flexi format enables you to look at NFκB binding at sites of your own design. This Kit works just like the other TransAM Kits, but you generate your own biotinylated oligo to capture activated NFκB binding. If you are interested in detecting NFκB family members binding to the traditional NFκB consensus sequence, please take a look at our other TransAM NFκB Kits.
TransAM™ Flexi Kits
TransAM Flexi Kits are perfect for researchers interested in studying alternative binding sites for their transcription factor. In TransAM Flexi, the researcher synthesizes a biotinylated oligo or PCR product, which contains the transcription factor-binding site of choice. A stimulated nuclear extract is mixed with this biotinylated oligo and then transferred to the wells of a 96-well streptavidin coated plate, where the biotinylated oligo containing the activated transcription factor is captured. Next, a primary antibody specific for an epitope on the bound and active form of the transcription factor is added followed by subsequent incubation with secondary antibody and Developing Solution to provide an easily quantified, sensitive colorimetric readout (Figure 1).
Figure 1: Flow chart of the TransAM Flexi process.
* Technology covered under EAT-filed patents and licensed to Active Motif. Use of TransAM in NFκB-related drug discovery may be covered under U.S. Patent No. 6,150,090 and require a license from Ariad Pharmaceuticals (Cambridge, MA, USA).
