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Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors involved in lipid transport and metabolism. As such, their roles in chronic diseases such as diabetes, obesity, atherosclerosis and cancer are heavily investigated. Transcriptional activity of PPARs is regulated by fatty acid binding. Three PPAR isotypes have been identified: α, β and γ. PPARγ stimulates lipolysis of circulating triglycerides and the subsequent uptake of fatty acids into adipose cells. PPARs can bind to DNA only as a heterodimer with the retinoid X receptor (RXR). TransAM PPARγ is provided with an antibody specific for the γ isotype when it is bound to its target DNA.
Contents & Storage
Each TransAM PPARγ Kit contains PPARγ primary antibody, HRP-conjugated secondary antibody, PPARγ wild-type and mutated oligonucleotides, THP-1 nuclear extract (positive control), Dithiothreitol, Protease Inhibitor Cocktail, Herring Sperm DNA, Lysis Buffer, Binding Buffer, 10X Washing Buffer, 10X Antibody Binding Buffer, Developing Solution, Stop Solution and one or five PPARγ 96-well assay plate(s) with plate sealer(s). Reagent storage conditions vary from room temperature to –80°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.
