YOUR CART
Sp1 was one of the first cellular transcription factors to be identified, cloned and characterized for its ability to bind to GC-boxes in regulatory promoter elements. Sp1 has always been described as a ubiquitous transcription factor that is required for the constitutive and inducible expression of a variety of genes, such as in the cell cycle and in mammalian development. Identification of new members of the Sp family, e.g. Sp2, Sp3, Sp4 and BTEB have redefined the understanding of Sp1-regulated genes. TransAM Sp1 and Sp1/Sp3 Kits contain antibodies specific for the active form of either Sp1 or Sp1/Sp3 when bound to its target DNA.
Contents & Storage
One or five Sp1 96-well assay plate(s) with plate sealer(s), Sp1 or Sp1 and Sp3 primary antibody(ies), HRP-conjugated secondary antibody, Sp1 wild-type and mutated oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, poly[d(I-C)], Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions. Reagent storage conditions vary from room temperature to -80°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.
