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Sp1 was one of the first cellular transcription factors to be identified, cloned and characterized for its ability to bind to GC-boxes in regulatory promoter elements. Sp1 has always been described as a ubiquitous transcription factor that is required for the constitutive and inducible expression of a variety of genes, such as in the cell cycle and in mammalian development. Identification of new members of the Sp family, e.g. Sp2, Sp3, Sp4 and BTEB have redefined the understanding of Sp1-regulated genes. TransAM Sp1 and Sp1/Sp3 Kits contain antibodies specific for the active form of either Sp1 or Sp1/Sp3 when bound to its target DNA.
Applications
TransAM Sp1 and Sp1/Sp3 Kits can be used in investigations of Sp1 pathophysiologies such as angiogenesis, metastasis and tumorigenesis.
- Sp1/Sp3 transcriptional activity regulation
- Protein structure/function studies of Sp1/Sp3
- Sp1/Sp3 activity modulation by oxidative stress
Figure 1: Monitoring Sp1 and Sp3 activity with the TransAM Sp1/Sp3 Kit.
Different amounts of untreated and H2O2-post treated nuclear extracts from MCF-7 cells are tested for Sp1 and Sp3 activity using the TransAM Sp1/Sp3 Kit.
