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Replication Protein A (RPA) is composed of RPA70, RPA32 and RPA14 (70, 32 and 14 kDa subunits, respectively). RPA binds directly to single-stranded DNA (ssDNA) where it organizes and protects ssDNA during DNA replication, recombination and repair. The inability to replicate or repair DNA breaks leads to cell cycle arrest and apoptosis.
Contents & Storage
One or five 96-well assay plates with plate sealers, RPA32 primary antibody, HRP-conjugated secondary antibody, competitor oligonucleotide, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffer, and Developing and Stop Solutions. Reagent storage conditions vary from room temperature to -80°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.
