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Replication Protein A (RPA) is composed of RPA70, RPA32 and RPA14 (70, 32 and 14 kDa subunits, respectively). RPA binds directly to single-stranded DNA (ssDNA) where it organizes and protects ssDNA during DNA replication, recombination and repair. The inability to replicate or repair DNA breaks leads to cell cycle arrest and apoptosis.
Applications
RPA is involved in the maintenance of genomic stability and therefore represents an excellent pharmacological target for developing drugs to treat cancer. Applications of the RPA DNA Repair Kit include:
- Profiling of RPA32 under various growth and stimulation conditions
- Drug screening and/or potency toward RPA
- RPA activity regulation
- Protein structure/function studies of RPA
Figure 1: Monitoring RPA32 binding with the RPA DNA Repair Kit.
Different amounts of nuclear extracts from unstimulated Raji and HeLa cells are tested for activity using the RPA DNA Repair Kit. These curves are provided for demonstration only.
