NR Sandwich PR ELISA
Progesterone is an essential regulator of the reproductive events associated with the establishment and maintenance of pregnancy, including ovulation and uterine & mammary gland development. Progesterone exerts its effects via the intracellular progesterone receptor (PR). PR is composed of two isoforms, A and B. The B form of PR acts as a transcriptional activator in different cellular contexts, whereas the A form functions as a strong inhibitor of transcriptional activity. Quantitative detection of PR levels is very important for predicting prognosis and evaluating the outcome of endocrine therapy in patients with breast and/or uterine cancer.
|NR Sandwich PR||1 x 96 rxns||49396||$650||Buy Now|
|5 x 96 rxns||49896||$2,750||Buy Now|
|NR Sandwich PR Manual|
|Nuclear Receptor Profile|
|Transcription Biology Products Brochure|
|MSDS: Sodium Azide|
|MSDS: Sulphuric Acid|
Figure 1: Monitoring protein expression levels of PR using NR Sandwich.
The NR Sandwich PR has many applications including:
- Study of PR transcriptional activity regulation
- Protein structure/function studies of PR and its mutated variants in areas such as osteoporosis, arteriosclerosis and breast cancer
Nuclear receptors (NR) belong to a superfamily of structurally related ligand-inducible transcription factors whose members include receptors for estrogen (ER), glucocorticoid (GR), androgen (AR), thyroid hormone (TR), progesterone (PR) and vitamin D (VDR). Inappropriate signaling by these receptors is associated with numerous disease states, including cancer, asthma, infertility, osteoporosis and arthritis.
Why use the Nuclear Receptor ELISAs?
- Quantitative results in just hours
- ELISA format eliminates the need to run, blot and develop gels
- NR Sandwich measures Total Nuclear Receptor
- Analyze multiple samples in low volume, high throughput
- Ability to assay both cellular extracts and recombinant proteins
The NR Sandwich ELISA method
In order to fully examine the activation of nuclear receptors, it is important to be able to quantify the total levels of a given nuclear receptor within a sample. Traditionally, total nuclear receptor protein levels have been monitored using Western blot, EMSA and reporter assays, which can be time-consuming and difficult to quantify. Active Motif's NR Sandwich Kits provide a simple, rapid and quantitative alternative to traditional low-throughput, labor-intensive assays. The NR Sandwich ELISAs use two antibodies that recognize different epitopes on the protein of interest. The Capture Antibody, which is supplied immobilized in the wells of an ELISA plate, binds the nuclear receptor protein when the sample is added. The Detecting Antibody then binds to the captured nuclear receptor protein. Subsequent incubation with a HRP-conjugated secondary antibody provides an easily quantified readout of total nuclear receptor protein levels (Figure 1).
Figure 1: NR Sandwich ELISA schematic.
Contents & Storage
One or five 96-well plate(s) coated with Capture Antibody, detecting antibody, HRP-conjugated secondary antibody, positive control cell extract and reaction buffers. Reagent storage conditions vary from room temperature to -80°C, see manual for details. All reagents are guaranteed stable for 6 months.